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10.1117/1.JBO.21.4.046005 http://scihub22266oqcxt.onion/10.1117/1.JBO.21.4.046005 C4833856!4833856!27086689     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biomed+Opt 2016 ; 21 (4): ä Nephropedia Template TP {{tp|p=27086689|t=2016. Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy |pdf=|usr=}}{{27086689}} gab.com Text Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy JO: J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=27086689 #FOAvip Macrophages adopt a variety of phenotypes that are a reflection of the many functions they perform as part of the immune system. In particular, metabolism is a phenotypic trait that differs between classically activated, proinflammatory macrophages, and alternatively activated, prohealing macrophages. Inflammatory macrophages have a metabolism based on glycolysis while alternatively activated macrophages generally rely on oxidative phosphorylation to generate chemical energy. We employ this shift in metabolism as an endogenous marker to identify the phenotype of individual macrophages via live-cell fluorescence lifetime imaging microscopy (FLIM). We demonstrate that polarized macrophages can be readily discriminated with the aid of a phasor approach to FLIM, which provides a fast and model-free method for analyzing fluorescence lifetime images. Twit Text FOAVip Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy ????J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=27086689 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27086689 #FOAvip English Wikipedia <ref>{{Cite pmid|27086689}}</ref> Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy #MMPMID27086689 Alfonso-García A; Smith TD; Datta R; Luu TU; Gratton E; Potma EO; Liu WF J Biomed Opt 2016[Apr]; 21 (4): ä PMID27086689show ga Macrophages adopt a variety of phenotypes that are a reflection of the many functions they perform as part of the immune system. In particular, metabolism is a phenotypic trait that differs between classically activated, proinflammatory macrophages, and alternatively activated, prohealing macrophages. Inflammatory macrophages have a metabolism based on glycolysis while alternatively activated macrophages generally rely on oxidative phosphorylation to generate chemical energy. We employ this shift in metabolism as an endogenous marker to identify the phenotype of individual macrophages via live-cell fluorescence lifetime imaging microscopy (FLIM). We demonstrate that polarized macrophages can be readily discriminated with the aid of a phasor approach to FLIM, which provides a fast and model-free method for analyzing fluorescence lifetime images. äLabel-free identification of macrophage phenotype by fluorescence life(epmc).pdf DeepDyve Pubget Overpricing