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2016 ; 6
(ä): 24431
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The first see-through frog created by breeding: description, inheritance
patterns, and dermal chromatophore structure
#MMPMID27080918
Sumida M
; Islam MM
; Igawa T
; Kurabayashi A
; Furukawa Y
; Sano N
; Fujii T
; Yoshizaki N
Sci Rep
2016[Apr]; 6
(ä): 24431
PMID27080918
show ga
We have succeeded in creating see-through frogs from natural color mutants of the
Japanese brown frog Rana japonica, which usually possesses an ochre or brown
back; this coloration enables the organs, blood vessels, and eggs to be observed
through the skin without performing dissection. We crossed two kinds of recessive
color mutant (black-eyed and gray-eyed) frogs through artificial insemination,
and F2 offspring produced frogs whose skin is translucent throughout the life
cycle. Three kinds of dermal chromatophores--xanthophores, iridophores, and
melanophores--are observed in a layered arrangement in the skin of wild-type
frogs, but few chromatophores were present in the skin of the see-through frogs.
The translucent skin enables observation of organ growth and cancer formation and
progression in the animal, which can be monitored over its entire life without
the need for dissection. See-through frogs thus provide a useful animal model for
environmental, medical, and biological research.