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Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Curr+Protoc+Mol+Biol 2016 ; 114 (ä): 20.11.1-20.11.22 Nephropedia Template TP
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Analysis of Individual Signaling Complexes by mMAPS, a Flow-proteometric System #MMPMID27038387
Chou CK; Tsou PH; Hsu JL; Lee HH; Wang YN; Kameoka J; Hung MC
Curr Protoc Mol Biol 2016[]; 114 (ä): 20.11.1-20.11.22 PMID27038387show ga
Signal transduction is essential for maintaining cells? normal physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for Multi-parameter Analysis of Proteins in a Single-complex or mMAPS for analyzing and quantifying target signaling complexes individually. mMAPS is a flow-proteometric-based system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissue samples or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe, requiring very low sample input.