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10.3892/ijmm.2016.2548 http://scihub22266oqcxt.onion/10.3892/ijmm.2016.2548 C4829143!4829143 !27049716     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27049716 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Int+J+Mol+Med 2016 ; 37 (5 ): 1239-46 Nephropedia Template TP {{tp|p=27049716 |t=2016. Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression |pdf=|usr=}}{{27049716 }} gab.com Text Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R phosphorylation and inhibiting matrix metalloproteinase-2 expression JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=27049716 #FOAvip The migration of vascular smooth muscle cells (VSMCs) may play a crucial role in the pathogenesis of vascular diseases, such as atherosclerosis and post-angioplasty restenosis. Platelet-derived growth factor (PDGF)-BB is a potent mitogen for VSMCs and plays an important role in the intimal accumulation of VSMCs. Magnobovatol, a new neolignan from the fruits of Magnolia obovata, has been shown to have anticancer properties. However, the effects of magnobovatol on VSMCs are unknown. In the present study, we examined the effects of magnobovatol on the PDGF?BB?induced migration of mouse and human VSMCs, as well as the underlying mechanisms. Magnobovatol significantly inhibited the PDGF?BB-induced migration of mouse and human VSMCs without inducing cell death (as shown by MTT assay and wound healing assay). Additionally, we demonstrated that magnobovatol significantly blocked the PDGF?BB-induced phosphorylation of the PDGF receptor (PDGF-R), Akt and extracellular signal?regulated kinase (ERK)1/2 by inhibiting the activation of the PDGF?BB signaling pathway. Moreover, in both mouse and human VSMCs, magnobovatol inhibited PDGF-induced matrix metalloproteinase (MMP)-2 expression at the mRNA and protein level, as well as the proteolytic activity of MMP-2 (as shown by western blot analysis, RT-PCR, gelatin zymography and ELISA). In addition, the sprout outgrowth formation of aortic rings induced by PDGF?BB was inhibited by magnobovatol (as shown by aortic ring assay). Taken together, our findings indicate that magnobovatol inhibits VSMC migration by decreasing MMP-2 expression through PDGF-R and the ERK1/2 and Akt pathways. Our data may improve the understanding of the anti-atherogenic effects of magnobovatol in VSMCs. Twit Text FOAVip Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R phosphorylation and inhibiting matrix metalloproteinase-2 expression ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=27049716 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27049716 #FOAvip English Wikipedia <ref>{{Cite pmid|27049716 }}</ref> Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF-R? phosphorylation and inhibiting matrix metalloproteinase-2 expression #MMPMID27049716 Kang H ; Ahn DH ; Pak JH ; Seo KH ; Baek NI ; Jang SW Int J Mol Med 2016[May]; 37 (5 ): 1239-46 PMID27049716 show ga The migration of vascular smooth muscle cells (VSMCs) may play a crucial role in the pathogenesis of vascular diseases, such as atherosclerosis and post-angioplasty restenosis. Platelet-derived growth factor (PDGF)-BB is a potent mitogen for VSMCs and plays an important role in the intimal accumulation of VSMCs. Magnobovatol, a new neolignan from the fruits of Magnolia obovata, has been shown to have anticancer properties. However, the effects of magnobovatol on VSMCs are unknown. In the present study, we examined the effects of magnobovatol on the PDGF?BB?induced migration of mouse and human VSMCs, as well as the underlying mechanisms. Magnobovatol significantly inhibited the PDGF?BB-induced migration of mouse and human VSMCs without inducing cell death (as shown by MTT assay and wound healing assay). Additionally, we demonstrated that magnobovatol significantly blocked the PDGF?BB-induced phosphorylation of the PDGF receptor (PDGF-R), Akt and extracellular signal?regulated kinase (ERK)1/2 by inhibiting the activation of the PDGF?BB signaling pathway. Moreover, in both mouse and human VSMCs, magnobovatol inhibited PDGF-induced matrix metalloproteinase (MMP)-2 expression at the mRNA and protein level, as well as the proteolytic activity of MMP-2 (as shown by western blot analysis, RT-PCR, gelatin zymography and ELISA). In addition, the sprout outgrowth formation of aortic rings induced by PDGF?BB was inhibited by magnobovatol (as shown by aortic ring assay). Taken together, our findings indicate that magnobovatol inhibits VSMC migration by decreasing MMP-2 expression through PDGF-R and the ERK1/2 and Akt pathways. Our data may improve the understanding of the anti-atherogenic effects of magnobovatol in VSMCs. |Anti-Inflammatory Agents/*pharmacology [MESH] |Aorta [MESH] |Cell Movement/drug effects [MESH] |Cell Proliferation/drug effects [MESH] |Cells, Cultured [MESH] |Humans [MESH] |Lignans/*pharmacology [MESH] |MAP Kinase Signaling System/drug effects [MESH] |Matrix Metalloproteinase 2/*metabolism [MESH] |Muscle, Smooth, Vascular/*cytology [MESH] |Myocytes, Smooth Muscle/*drug effects/*metabolism [MESH] |Phosphorylation [MESH] |Proteolysis [MESH] |Proto-Oncogene Proteins c-akt/metabolism [MESH] |Receptor, Platelet-Derived Growth Factor beta/*metabolism [MESH]Magnobovatol inhibits smooth muscle cell migration by suppressing PDGF(epmc).pdf DeepDyve Pubget Overpricing