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10.1002/0471142727.mb3004s114 http://scihub22266oqcxt.onion/10.1002/0471142727.mb3004s114 C4829120!4829120!27038389     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Curr+Protoc+Mol+Biol 2016 ; 114 (ä): 30.4.1-30.4.32 Nephropedia Template TP {{tp|p=27038389|t=2016. Metabolomics by Gas Chromatography-Mass Spectrometry: the combination of targeted and untargeted profiling |pdf=|usr=}}{{27038389}} gab.com Text Metabolomics by Gas Chromatography-Mass Spectrometry: the combination of targeted and untargeted profiling JO: Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27038389 #FOAvip Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is ideal for identifying and quantitating small molecular metabolites (<650 daltons), including small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins, often using chemical derivatization to make these compounds volatile enough for gas chromatography. This unit shows that on GC-MS- based metabolomics easily allows integrating targeted assays for absolute quantification of specific metabolites with untargeted metabolomics to discover novel compounds. Complemented by database annotations using large spectral libraries and validated, standardized standard operating procedures, GC-MS can identify and semi-quantify over 200 compounds per study in human body fluids (e.g., plasma, urine or stool) samples. Deconvolution software enables detection of more than 300 additional unidentified signals that can be annotated through accurate mass instruments with appropriate data processing workflows, similar to liquid chromatography-MS untargeted profiling (LC-MS). Hence, GC-MS is a mature technology that not only uses classic detectors (?quadrupole?) but also target mass spectrometers (?triple quadrupole?) and accurate mass instruments (?quadrupole-time of flight?). This unit covers the following aspects of GC-MS-based metabolomics: (i) sample preparation from mammalian samples, (ii) acquisition of data, (iii) quality control, and (iv) data processing. Twit Text FOAVip Metabolomics by Gas Chromatography-Mass Spectrometry: the combination of targeted and untargeted profiling ????Curr Protoc Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=27038389 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27038389 #FOAvip English Wikipedia <ref>{{Cite pmid|27038389}}</ref> Metabolomics by Gas Chromatography-Mass Spectrometry: the combination of targeted and untargeted profiling #MMPMID27038389 Fiehn O Curr Protoc Mol Biol 2016[]; 114 (ä): 30.4.1-30.4.32 PMID27038389show ga Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is ideal for identifying and quantitating small molecular metabolites (<650 daltons), including small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins, often using chemical derivatization to make these compounds volatile enough for gas chromatography. This unit shows that on GC-MS- based metabolomics easily allows integrating targeted assays for absolute quantification of specific metabolites with untargeted metabolomics to discover novel compounds. Complemented by database annotations using large spectral libraries and validated, standardized standard operating procedures, GC-MS can identify and semi-quantify over 200 compounds per study in human body fluids (e.g., plasma, urine or stool) samples. Deconvolution software enables detection of more than 300 additional unidentified signals that can be annotated through accurate mass instruments with appropriate data processing workflows, similar to liquid chromatography-MS untargeted profiling (LC-MS). Hence, GC-MS is a mature technology that not only uses classic detectors (?quadrupole?) but also target mass spectrometers (?triple quadrupole?) and accurate mass instruments (?quadrupole-time of flight?). This unit covers the following aspects of GC-MS-based metabolomics: (i) sample preparation from mammalian samples, (ii) acquisition of data, (iii) quality control, and (iv) data processing. äMetabolomics by Gas Chromatography-Mass Spectrometry: the combination (epmc).pdf DeepDyve Pubget Overpricing