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10.1016/j.cell.2016.02.045 http://scihub22266oqcxt.onion/10.1016/j.cell.2016.02.045 C4826323!4826323!27020755     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2016 ; 165 (2): 372-81 Nephropedia Template TP {{tp|p=27020755|t=2016. Splicing of nascent RNA coincides with intron exit from RNA polymerase II |pdf=|usr=}}{{27020755}} gab.com Text Splicing of nascent RNA coincides with intron exit from RNA polymerase II JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=27020755 #FOAvip Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and introns are removed from pre-mRNA by the spliceosome. Understanding the time lag between Pol II progression and splicing could provide mechanistic insights into the regulation of gene expression. Here we present two single molecule nascent RNA sequencing methods that directly determine the progress of splicing catalysis as a function of Pol II position. Endogenous genes were analyzed on a global scale in budding yeast. We show that splicing is 50% complete when Pol II is only 45nt downstream of introns, with the first spliced products observed as introns emerge from Pol II. Perturbations that slow the rate of spliceosome assembly or speed up the rate of transcription caused splicing delays, showing that regulation of both processes determines in vivo splicing profiles. We propose that matched rates streamline the gene expression pathway, while allowing regulation through kinetic competition. Twit Text FOAVip Splicing of nascent RNA coincides with intron exit from RNA polymerase II ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=27020755 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27020755 #FOAvip English Wikipedia <ref>{{Cite pmid|27020755}}</ref> Splicing of nascent RNA coincides with intron exit from RNA polymerase II #MMPMID27020755 Oesterreich FC; Herzel L; Straube K; Hujer K; Howard J; Neugebauer KM Cell 2016[Apr]; 165 (2): 372-81 PMID27020755show ga Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and introns are removed from pre-mRNA by the spliceosome. Understanding the time lag between Pol II progression and splicing could provide mechanistic insights into the regulation of gene expression. Here we present two single molecule nascent RNA sequencing methods that directly determine the progress of splicing catalysis as a function of Pol II position. Endogenous genes were analyzed on a global scale in budding yeast. We show that splicing is 50% complete when Pol II is only 45nt downstream of introns, with the first spliced products observed as introns emerge from Pol II. Perturbations that slow the rate of spliceosome assembly or speed up the rate of transcription caused splicing delays, showing that regulation of both processes determines in vivo splicing profiles. We propose that matched rates streamline the gene expression pathway, while allowing regulation through kinetic competition. äSplicing of nascent RNA coincides with intron exit from RNA polymerase(epmc).pdf DeepDyve Pubget Overpricing