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10.1073/pnas.1602488113 http://scihub22266oqcxt.onion/10.1073/pnas.1602488113 C4822615!4822615 !26979955     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26979955 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Proc+Natl+Acad+Sci+U+S+A 2016 ; 113 (13 ): E1890-7 Nephropedia Template TP {{tp|p=26979955 |t=2016. Detection, phenotyping, and quantification of antigen-specific T cells using a peptide-MHC dodecamer |pdf=|usr=}}{{26979955 }} gab.com Text Detection, phenotyping, and quantification of antigen-specific T cells using a peptide-MHC dodecamer JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26979955 #FOAvip Here we report a peptide-MHC (pMHC) dodecamer as a "next generation" technology that is a significantly more sensitive and versatile alternative to pMHC tetramers for the detection, isolation, and phenotypic analysis of antigen-specific T cells. In particular, dodecamers are able to detect two- to fivefold more antigen-specific T cells in both human and murine CD4(+)and CD8(+)?? T-cell compartments compared with the equivalent tetramers. The low-affinity, tetramer-negative, dodecamer-positive T cells showed comparable effector cytokine responses as those of high-affinity, tetramer-positive T cells. Dodecamers are able to detect early stage CD4(+)CD8(+)double-positive thymocytes on which T-cell receptors are 10- to 30-fold less dense than mature T cells. Dodecamers also show utility in the analysis of ?? T cells and in cytometry by time-of-flight applications. This construct has a simple structure with a central scaffold protein linked to four streptavidin molecules, each having three pMHC ligands or other molecules. The dodecamer is straightforward and inexpensive to produce and is compatible with current tetramer technology and commercially available streptavidin conjugates. Twit Text FOAVip Detection, phenotyping, and quantification of antigen-specific T cells using a peptide-MHC dodecamer ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26979955 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26979955 #FOAvip English Wikipedia <ref>{{Cite pmid|26979955 }}</ref> Detection, phenotyping, and quantification of antigen-specific T cells using a peptide-MHC dodecamer #MMPMID26979955 Huang J ; Zeng X ; Sigal N ; Lund PJ ; Su LF ; Huang H ; Chien YH ; Davis MM Proc Natl Acad Sci U S A 2016[Mar]; 113 (13 ): E1890-7 PMID26979955 show ga Here we report a peptide-MHC (pMHC) dodecamer as a "next generation" technology that is a significantly more sensitive and versatile alternative to pMHC tetramers for the detection, isolation, and phenotypic analysis of antigen-specific T cells. In particular, dodecamers are able to detect two- to fivefold more antigen-specific T cells in both human and murine CD4(+)and CD8(+)?? T-cell compartments compared with the equivalent tetramers. The low-affinity, tetramer-negative, dodecamer-positive T cells showed comparable effector cytokine responses as those of high-affinity, tetramer-positive T cells. Dodecamers are able to detect early stage CD4(+)CD8(+)double-positive thymocytes on which T-cell receptors are 10- to 30-fold less dense than mature T cells. Dodecamers also show utility in the analysis of ?? T cells and in cytometry by time-of-flight applications. This construct has a simple structure with a central scaffold protein linked to four streptavidin molecules, each having three pMHC ligands or other molecules. The dodecamer is straightforward and inexpensive to produce and is compatible with current tetramer technology and commercially available streptavidin conjugates. |Animals [MESH] |CD4-Positive T-Lymphocytes/*immunology/parasitology [MESH] |CD8-Positive T-Lymphocytes/*immunology/physiology [MESH] |Flow Cytometry/methods [MESH] |Humans [MESH] |Immunophenotyping/*methods [MESH] |Major Histocompatibility Complex [MESH] |Mice, Transgenic [MESH] |Peptides/chemistry/*metabolism [MESH] |Receptors, Antigen, T-Cell/genetics/metabolism [MESH] |Single-Cell Analysis/methods [MESH]Detection, phenotyping, and quantification of antigen-specific T cells(epmc).pdf DeepDyve Pubget Overpricing