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10.1002/chem.201502934

http://scihub22266oqcxt.onion/10.1002/chem.201502934
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C4816641!4816641!26420633
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suck abstract from ncbi


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pmid26420633      Chemistry 2015 ; 21 (46): 16359-63
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  • Fluorescence polarization-based nucleic acid testing for rapid and cost-effective diagnosis of infectious disease #MMPMID26420633
  • Park KS; Charles RC; Ryan ET; Weissleder R; Lee H
  • Chemistry 2015[Nov]; 21 (46): 16359-63 PMID26420633show ga
  • A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: (i) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; (ii) universal reporters conjugated with a single fluorophore; and (iii) fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in relatively short periods of time.
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