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10.1002/chem.201502934

http://scihub22266oqcxt.onion/10.1002/chem.201502934
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C4816641!4816641 !26420633
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suck abstract from ncbi


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pmid26420633
      Chemistry 2015 ; 21 (46 ): 16359-63
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  • Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease #MMPMID26420633
  • Park KS ; Charles RC ; Ryan ET ; Weissleder R ; Lee H
  • Chemistry 2015[Nov]; 21 (46 ): 16359-63 PMID26420633 show ga
  • A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1)?detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2)?universal reporters conjugated with a single fluorophore; and 3)?fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
  • |Base Sequence [MESH]
  • |Biosensing Techniques/*methods [MESH]
  • |Communicable Diseases/*diagnosis [MESH]
  • |Cost-Benefit Analysis [MESH]
  • |DNA-Directed DNA Polymerase/*chemistry/metabolism [MESH]
  • |Fluorescence Polarization [MESH]
  • |Fluorescent Dyes/*chemistry [MESH]
  • |Nucleic Acid Amplification Techniques/*economics/*methods [MESH]
  • |Nucleic Acids/analysis/*chemistry/metabolism [MESH]


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