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10.1002/pro.2861 http://scihub22266oqcxt.onion/10.1002/pro.2861 C4815408!4815408!26660914     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26660914&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Protein+Sci 2016 ; 25 (3): 720-33 Nephropedia Template TP {{tp|p=26660914|t=2016. Protein purification and crystallization artifacts: The tale usually not told |pdf=|usr=}}{{26660914}} gab.com Text Protein purification and crystallization artifacts: The tale usually not told JO: Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=26660914 #FOAvip The misidentification of a protein sample, or contamination of a sample with the wrong protein, may be a potential reason for the non?reproducibility of experiments. This problem may occur in the process of heterologous overexpression and purification of recombinant proteins, as well as purification of proteins from natural sources. If the contaminated or misidentified sample is used for crystallization, in many cases the problem may not be detected until structures are determined. In the case of functional studies, the problem may not be detected for years. Here several procedures that can be successfully used for the identification of crystallized protein contaminants, including: (i) a lattice parameter search against known structures, (ii) sequence or fold identification from partially built models, and (iii) molecular replacement with common contaminants as search templates have been presented. A list of common contaminant structures to be used as alternative search models was provided. These methods were used to identify four cases of purification and crystallization artifacts. This report provides troubleshooting pointers for researchers facing difficulties in phasing or model building. Twit Text FOAVip Protein purification and crystallization artifacts: The tale usually not told ????Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=26660914 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26660914 #FOAvip English Wikipedia <ref>{{Cite pmid|26660914}}</ref> Protein purification and crystallization artifacts: The tale usually not told #MMPMID26660914 Niedzialkowska E; Gasiorowska O; Handing KB; Majorek KA; Porebski PJ; Shabalin IG; Zasadzinska E; Cymborowski M; Minor W Protein Sci 2016[Mar]; 25 (3): 720-33 PMID26660914show ga The misidentification of a protein sample, or contamination of a sample with the wrong protein, may be a potential reason for the non?reproducibility of experiments. This problem may occur in the process of heterologous overexpression and purification of recombinant proteins, as well as purification of proteins from natural sources. If the contaminated or misidentified sample is used for crystallization, in many cases the problem may not be detected until structures are determined. In the case of functional studies, the problem may not be detected for years. Here several procedures that can be successfully used for the identification of crystallized protein contaminants, including: (i) a lattice parameter search against known structures, (ii) sequence or fold identification from partially built models, and (iii) molecular replacement with common contaminants as search templates have been presented. A list of common contaminant structures to be used as alternative search models was provided. These methods were used to identify four cases of purification and crystallization artifacts. This report provides troubleshooting pointers for researchers facing difficulties in phasing or model building. äProtein purification and crystallization artifacts: The tale usually n(epmc).pdf DeepDyve Pubget Overpricing