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10.1038/srep23821 http://scihub22266oqcxt.onion/10.1038/srep23821 C4814874!4814874 !27030642     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\27030642 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2016 ; 6 (ä): 23821 Nephropedia Template TP {{tp|p=27030642 |t=2016. CD4(+) and CD8(+) TCR? repertoires possess different potentials to generate extraordinarily high-avidity T cells |pdf=|usr=}}{{27030642 }} gab.com Text CD4(+) and CD8(+) TCR repertoires possess different potentials to generate extraordinarily high-avidity T cells JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27030642 #FOAvip Recent high throughput sequencing analysis has revealed that the TCR? repertoire is largely different between CD8(+) and CD4(+) T cells. Here, we show that the transduction of SIG35?, the public chain-centric HLA-A*02:01(A2)/MART127-35 TCR? hemichain, conferred A2/MART127-35 reactivity to a substantial subset of both CD8(+) and CD4(+) T cells regardless of their HLA-A2 positivity. T cells individually reconstituted with SIG35? and different A2/MART127-35 TCR? genes isolated from CD4(+) or CD8(+) T cells exhibited a wide range of avidity. Surprisingly, approximately half of the A2/MART127-35 TCRs derived from CD4(+) T cells, but none from CD8(+) T cells, were stained by A2/MART127-35 monomer and possessed broader cross-reactivity. Our results suggest that the differences in the primary structure of peripheral CD4(+) and CD8(+) TCR? repertoire indeed result in the differences in their ability to form extraordinarily high avidity T cells which would otherwise have been deleted by central tolerance. Twit Text FOAVip CD4(+) and CD8(+) TCR repertoires possess different potentials to generate extraordinarily high-avidity T cells ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=27030642 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27030642 #FOAvip English Wikipedia <ref>{{Cite pmid|27030642 }}</ref> CD4(+) and CD8(+) TCR? repertoires possess different potentials to generate extraordinarily high-avidity T cells #MMPMID27030642 Nakatsugawa M ; Rahman MA ; Yamashita Y ; Ochi T ; Wnuk P ; Tanaka S ; Chamoto K ; Kagoya Y ; Saso K ; Guo T ; Anczurowski M ; Butler MO ; Hirano N Sci Rep 2016[Mar]; 6 (ä): 23821 PMID27030642 show ga Recent high throughput sequencing analysis has revealed that the TCR? repertoire is largely different between CD8(+) and CD4(+) T cells. Here, we show that the transduction of SIG35?, the public chain-centric HLA-A*02:01(A2)/MART127-35 TCR? hemichain, conferred A2/MART127-35 reactivity to a substantial subset of both CD8(+) and CD4(+) T cells regardless of their HLA-A2 positivity. T cells individually reconstituted with SIG35? and different A2/MART127-35 TCR? genes isolated from CD4(+) or CD8(+) T cells exhibited a wide range of avidity. Surprisingly, approximately half of the A2/MART127-35 TCRs derived from CD4(+) T cells, but none from CD8(+) T cells, were stained by A2/MART127-35 monomer and possessed broader cross-reactivity. Our results suggest that the differences in the primary structure of peripheral CD4(+) and CD8(+) TCR? repertoire indeed result in the differences in their ability to form extraordinarily high avidity T cells which would otherwise have been deleted by central tolerance. |Amino Acid Sequence [MESH] |CD4-Positive T-Lymphocytes/cytology/*immunology [MESH] |CD8-Positive T-Lymphocytes/cytology/*immunology [MESH] |Cross Reactions [MESH] |Gene Expression [MESH] |HLA-A2 Antigen/genetics/immunology [MESH] |Humans [MESH] |MART-1 Antigen/genetics/immunology/*metabolism [MESH] |Primary Cell Culture [MESH] |Receptors, Antigen, T-Cell, alpha-beta/genetics/*immunology [MESH]CD4(+) and CD8(+) TCR? repertoires possess different potentials to gen(epmc).pdf DeepDyve Pubget Overpricing