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2016 ; 2016
(ä): 9618480
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Skeletal Muscle-Derived Stem/Progenitor Cells: A Potential Strategy for the
Treatment of Acute Kidney Injury
#MMPMID27069485
Pavyde E
; Maciulaitis R
; Mauricas M
; Sudzius G
; Ivanauskaite Didziokiene E
; Laurinavicius A
; Sutkeviciene N
; Stankevicius E
; Maciulaitis J
; Usas A
Stem Cells Int
2016[]; 2016
(ä): 9618480
PMID27069485
show ga
Skeletal muscle-derived stem/progenitor cells (MDSPCs) have been thoroughly
investigated and already used in preclinical studies. However, therapeutic
potential of MDSPCs isolated using preplate isolation technique for acute kidney
injury (AKI) has not been evaluated. We aimed to characterize rat MDSPCs, compare
them with bone marrow mesenchymal stem cells (BM-MSCs), and evaluate the
feasibility of MDSPCs therapy for gentamicin-induced AKI in rats. We have
isolated and characterized rat MDSPCs and BM-MSCs. Characteristics of rat BM-MSCs
and MDSPCs were assessed by population doubling time, flow cytometry,
immunofluorescence staining, RT-PCR, and multipotent differentiation capacity.
Gentamicin-induced AKI model in rat was used to examine MDSPCs therapeutic
effect. Physiological and histological kidney parameters were determined. MDSPCs
exhibited similar immunophenotype, stem cell gene expression, and multilineage
differentiation capacities as BM-MSCs, but they demonstrated higher proliferation
rate. Single intravenous MDSPCs injection accelerated functional and
morphological kidney recovery, as reflected by significantly lower serum
creatinine levels, renal injury score, higher urinary creatinine, and GFR levels.
PKH-26-labeled MDSPCs were identified within renal cortex 1 and 2 weeks after
cell administration, indicating MDSPCs capacity to migrate and populate renal
tissue. In conclusion, MDSPCs are capable of mediating functional and
histological kidney recovery and can be considered as potential strategy for AKI
treatment.
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