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10.3892/etm.2016.3027 http://scihub22266oqcxt.onion/10.3892/etm.2016.3027 C4812120!4812120 !27073458     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27073458 &cmd=llinks): Failed to open stream: HTTP request failed! 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Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-?1 expression |pdf=|usr=}}{{27073458 }} gab.com Text Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF- 1 expression JO: Exp Ther Med http://www.kidney.de/pget.php?&tw=1&pmid=27073458 #FOAvip The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ-S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ-S and fosinopril-containing serum (For-S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, ?-catenin and transforming growth factor (TGF)-?1 in MCs. The results indicated that YQ-S inhibited LPS-induced MC proliferation. The Wnt4 and TGF-?1 mRNA expression levels were reduced in the YQ-S group (P<0.01 or P<0.05). Furthermore, the Wnt4, ?-catenin and TGF-?1 protein expression levels were suppressed in the YQ-S group (P<0.01 or P<0.05). Therefore, YQ-S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-?1 expression. Twit Text FOAVip Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF- 1 expression ????Exp Ther Med http://www.kidney.de/pget.php?&tw=1&pmid=27073458 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27073458 #FOAvip English Wikipedia <ref>{{Cite pmid|27073458 }}</ref> Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-?1 expression #MMPMID27073458 Yang L ; Sun X ; Zhan Y ; Liu H ; Wen Y ; Mao H ; Dong XI ; Li P Exp Ther Med 2016[Apr]; 11 (4 ): 1410-1416 PMID27073458 show ga The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ-S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ-S and fosinopril-containing serum (For-S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, ?-catenin and transforming growth factor (TGF)-?1 in MCs. The results indicated that YQ-S inhibited LPS-induced MC proliferation. The Wnt4 and TGF-?1 mRNA expression levels were reduced in the YQ-S group (P<0.01 or P<0.05). Furthermore, the Wnt4, ?-catenin and TGF-?1 protein expression levels were suppressed in the YQ-S group (P<0.01 or P<0.05). Therefore, YQ-S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-?1 expression. äYi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced(epmc).pdf DeepDyve Pubget Overpricing