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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Clin+Microbiol
2016 ; 54
(4
): 1094-100
Nephropedia Template TP
gab.com Text
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English Wikipedia
Evaluation of Luminex xTAG Gastrointestinal Pathogen Panel Assay for Detection of
Multiple Diarrheal Pathogens in Fecal Samples in Vietnam
#MMPMID26865681
Duong VT
; Phat VV
; Tuyen HT
; Dung TT
; Trung PD
; Minh PV
; Tu le TP
; Campbell JI
; Le Phuc H
; Ha TT
; Ngoc NM
; Huong NT
; Tam PT
; Huong DT
; Xang NV
; Dong N
; Phuong le T
; Hung NV
; Phu BD
; Phuc TM
; Thwaites GE
; Vi LL
; Rabaa MA
; Thompson CN
; Baker S
J Clin Microbiol
2016[Apr]; 54
(4
): 1094-100
PMID26865681
show ga
Diarrheal disease is a complex syndrome that remains a leading cause of global
childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely
and precise manner is important for making treatment decisions and informing
public health policy, but accurate diagnosis is a major challenge in
industrializing countries. Multiplex molecular diagnostic techniques may
represent a significant improvement over classical approaches. We evaluated the
Luminex xTAG gastrointestinal pathogen panel (GPP) assay for the detection of
common enteric bacterial and viral pathogens in Vietnam. Microbiological culture
and real-time PCR were used as gold standards. The tests were performed on 479
stool samples collected from people admitted to the hospital for diarrheal
disease throughout Vietnam. Sensitivity and specificity were calculated for the
xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and
specificity for the xTAG GPP were >88% for Shigellaspp.,Campylobacterspp.,
rotavirus, norovirus genotype 1/2 (GI/GII), and adenovirus compared to those of
microbiological culture and/or real-time PCR. However, the specificity was low
(?60%) for Salmonella species. Additionally, a number of important pathogens that
are not identified in routine hospital procedures in this setting, such as
Cryptosporidiumspp. and Clostridium difficile, were detected with the GPP. The
use of the Luminex xTAG GPP for the detection of enteric pathogens in settings,
like Vietnam, would dramatically improve the diagnostic accuracy and capacity of
hospital laboratories, allowing for timely and appropriate therapy decisions and
a wider understanding of the epidemiology of pathogens associated with severe
diarrheal disease in low-resource settings.