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Propionibacterium acnes: Disease-Causing Agent or Common Contaminant? Detection
in Diverse Patient Samples by Next-Generation Sequencing
#MMPMID26818667
Mollerup S
; Friis-Nielsen J
; Vinner L
; Hansen TA
; Richter SR
; Fridholm H
; Herrera JA
; Lund O
; Brunak S
; Izarzugaza JM
; Mourier T
; Nielsen LP
; Hansen AJ
J Clin Microbiol
2016[Apr]; 54
(4
): 980-7
PMID26818667
show ga
Propionibacterium acnesis the most abundant bacterium on human skin, particularly
in sebaceous areas.P. acnesis suggested to be an opportunistic pathogen involved
in the development of diverse medical conditions but is also a proven contaminant
of human clinical samples and surgical wounds. Its significance as a pathogen is
consequently a matter of debate. In the present study, we investigated the
presence ofP. acnesDNA in 250 next-generation sequencing data sets generated from
180 samples of 20 different sample types, mostly of cancerous origin. The samples
were subjected to either microbial enrichment, involving nuclease treatment to
reduce the amount of host nucleic acids, or shotgun sequencing. We detected high
proportions ofP. acnesDNA in enriched samples, particularly skin tissue-derived
and other tissue samples, with the levels being higher in enriched samples than
in shotgun-sequenced samples.P. acnesreads were detected in most samples
analyzed, though the proportions in most shotgun-sequenced samples were low. Our
results show thatP. acnescan be detected in practically all sample types when
molecular methods, such as next-generation sequencing, are employed. The
possibility of contamination from the patient or other sources, including
laboratory reagents or environment, should therefore always be considered
carefully whenP. acnesis detected in clinical samples. We advocate that detection
ofP. acnesalways be accompanied by experiments validating the association between
this bacterium and any clinical condition.
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