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10.1186/s13059-016-0915-2 http://scihub22266oqcxt.onion/10.1186/s13059-016-0915-2 C4807595!4807595!27013184     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Biol 2016 ; 17 (ä): ä Nephropedia Template TP {{tp|p=27013184|t=2016. CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries |pdf=|usr=}}{{27013184}} gab.com Text CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries JO: Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=27013184 #FOAvip Background: Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes. Results: Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide RNA (sgRNA) libraries for all organisms with annotated genomes. CLD is suitable for the design of libraries using modified CRISPR enzymes and targeting non-coding regions. To demonstrate its utility, we perform a pooled screen for modulators of the TNF-related apoptosis inducing ligand (TRAIL) pathway using a custom library of 12,471 sgRNAs. Conclusion: CLD predicts a high fraction of functional sgRNAs and is publicly available at https://github.com/boutroslab/cld. Electronic supplementary material: The online version of this article (doi:10.1186/s13059-016-0915-2) contains supplementary material, which is available to authorized users. Twit Text FOAVip CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries ????Genome Biol http://www.kidney.de/pget.php?&tw=1&pmid=27013184 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27013184 #FOAvip English Wikipedia <ref>{{Cite pmid|27013184}}</ref> CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries #MMPMID27013184 Heigwer F; Zhan T; Breinig M; Winter J; Brügemann D; Leible S; Boutros M Genome Biol 2016[]; 17 (ä): ä PMID27013184show ga Background: Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes. Results: Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide RNA (sgRNA) libraries for all organisms with annotated genomes. CLD is suitable for the design of libraries using modified CRISPR enzymes and targeting non-coding regions. To demonstrate its utility, we perform a pooled screen for modulators of the TNF-related apoptosis inducing ligand (TRAIL) pathway using a custom library of 12,471 sgRNAs. Conclusion: CLD predicts a high fraction of functional sgRNAs and is publicly available at https://github.com/boutroslab/cld. Electronic supplementary material: The online version of this article (doi:10.1186/s13059-016-0915-2) contains supplementary material, which is available to authorized users. äCRISPR library designer (CLD): software for multispecies design of sin(epmc).pdf DeepDyve Pubget Overpricing