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2015 ; 2
(12
): 150414
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Are microbes fundamentally different than macroorganisms? Convergence and a
possible case for neutral phenotypic evolution in testate amoeba (Amoebozoa:
Arcellinida)
#MMPMID27019725
Oliverio AM
; Lahr DJ
; Grant J
; Katz LA
R Soc Open Sci
2015[Dec]; 2
(12
): 150414
PMID27019725
show ga
This study reveals extensive phenotypic convergence based on the non-monophyly of
genera and morphospecies of testate (shelled) amoebae. Using two independent
markers, small subunit ribosomal DNA (ssu-rDNA) and mitochondrial cytochrome
oxidase I (COI), we demonstrate discordance between morphology and molecules for
'core Nebela' species (Arcellinida; Amoebozoa). Prior work using just a single
locus, ssu-rDNA, also supported the non-monophyly of the genera Hyalosphenia and
Nebela as well as for several morphospecies within these genera. Here, we
obtained COI gene sequences of 59 specimens from seven morphospecies and ssu-rDNA
gene sequences of 50 specimens from six morphospecies of hyalosphenids. Our
analyses corroborate the prior ssu-rDNA findings of morphological convergence in
test (shell) morphologies, as COI and ssu-rDNA phylogenies are concordant.
Further, the monophyly of morphospecies is rejected using approximately unbiased
tests. Given that testate amoebae are used as bioindicators in both
palaeoecological and contemporary studies of threatened ecosystems such as bogs
and fens, understanding the discordance between morphology and genetics in the
hyalosphenids is essential for interpretation of indicator species. Further,
while convergence is normally considered the result of natural selection, it is
possible that neutrality underlies phenotypic evolution in these microorganisms.