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10.1038/nprot.2015.010 http://scihub22266oqcxt.onion/10.1038/nprot.2015.010 C4806852!4806852!25551665     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Protoc 2015 ; 10 (1): 205-15 Nephropedia Template TP {{tp|p=25551665|t=2015. A high-throughput in vivo micronucleus assay for genome instability screening in mice |pdf=|usr=}}{{25551665}} gab.com Text A high-throughput in vivo micronucleus assay for genome instability screening in mice JO: Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25551665 #FOAvip We describe a sensitive, robust, high-throughput method for quantifying the formation of micronuclei, markers of genome instability, in mouse erythrocytes. Micronuclei are whole chromosomes or chromosome segments that have been separated from the nucleus. Other methods of detection rely on labour-intensive, microscopy-based techniques. Here, we describe a 2-d, 96-well plate-based flow cytometric method of micronucleus scoring that is simple enough for a research technician experienced in flow cytometry to perform. The assay detects low levels of genome instability that cannot be readily identified by classic phenotyping, using 25 ?l of blood. By using this assay, we have screened >10,000 blood samples and discovered novel genes that contribute to vertebrate genome maintenance, as well as novel disease models and mechanisms of genome instability disorders. We discuss experimental design considerations, including statistical power calculation, we provide troubleshooting tips, and we discuss factors that contribute to a false-positive increase in the number of micronucleated red blood cells and to experimental variability. Twit Text FOAVip A high-throughput in vivo micronucleus assay for genome instability screening in mice ????Nat Protoc http://www.kidney.de/pget.php?&tw=1&pmid=25551665 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25551665 #FOAvip English Wikipedia <ref>{{Cite pmid|25551665}}</ref> A high-throughput in vivo micronucleus assay for genome instability screening in mice #MMPMID25551665 Balmus G; Karp NA; Ng BL; Jackson SP; Adams DJ; McIntyre RE Nat Protoc 2015[Jan]; 10 (1): 205-15 PMID25551665show ga We describe a sensitive, robust, high-throughput method for quantifying the formation of micronuclei, markers of genome instability, in mouse erythrocytes. Micronuclei are whole chromosomes or chromosome segments that have been separated from the nucleus. Other methods of detection rely on labour-intensive, microscopy-based techniques. Here, we describe a 2-d, 96-well plate-based flow cytometric method of micronucleus scoring that is simple enough for a research technician experienced in flow cytometry to perform. The assay detects low levels of genome instability that cannot be readily identified by classic phenotyping, using 25 ?l of blood. By using this assay, we have screened >10,000 blood samples and discovered novel genes that contribute to vertebrate genome maintenance, as well as novel disease models and mechanisms of genome instability disorders. We discuss experimental design considerations, including statistical power calculation, we provide troubleshooting tips, and we discuss factors that contribute to a false-positive increase in the number of micronucleated red blood cells and to experimental variability. äA high-throughput in vivo micronucleus assay for genome instability sc(epmc).pdf DeepDyve Pubget Overpricing