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10.1101/pdb.top086892 http://scihub22266oqcxt.onion/10.1101/pdb.top086892 C4804892!4804892!26933254     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cold+Spring+Harb+Protoc 2016 ; 2016 (3): pdb.top086892 Nephropedia Template TP {{tp|p=26933254|t=2016. Large-Scale Single-Guide RNA Library Construction and Use for Genetic Screens: CRISPR/Cas9-based Genetic Screens |pdf=|usr=}}{{26933254}} gab.com Text Large-Scale Single-Guide RNA Library Construction and Use for Genetic Screens: CRISPR/Cas9-based Genetic Screens JO: Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=26933254 #FOAvip The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system enables efficient targeting of large numbers of genes through the use of single-guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockout mutants can be readily generated via transduction of Cas9/sgRNA lentiviral pools, screened for a phenotype of interest, and tracked using high-throughput DNA sequencing. This technique represents the first general method for undertaking systematic loss-of-function genetic screens in mammalian cells. In this chapter, we outline the steps for conducting CRISPR-based screens from the initial library design to final data analysis and provide guidelines for developing an appropriate screening strategy. Twit Text FOAVip Large-Scale Single-Guide RNA Library Construction and Use for Genetic Screens: CRISPR/Cas9-based Genetic Screens ????Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=26933254 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26933254 #FOAvip English Wikipedia <ref>{{Cite pmid|26933254}}</ref> Large-Scale Single-Guide RNA Library Construction and Use for Genetic Screens: CRISPR/Cas9-based Genetic Screens #MMPMID26933254 Wang T; Lander ES; Sabatini DM Cold Spring Harb Protoc 2016[]; 2016 (3): pdb.top086892 PMID26933254show ga The ability to systematically disrupt genes serves as a powerful tool for understanding their function. The programmable Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system enables efficient targeting of large numbers of genes through the use of single-guide RNA (sgRNA) libraries. In cultured mammalian cells, collections of knockout mutants can be readily generated via transduction of Cas9/sgRNA lentiviral pools, screened for a phenotype of interest, and tracked using high-throughput DNA sequencing. This technique represents the first general method for undertaking systematic loss-of-function genetic screens in mammalian cells. In this chapter, we outline the steps for conducting CRISPR-based screens from the initial library design to final data analysis and provide guidelines for developing an appropriate screening strategy. äLarge-Scale Single-Guide RNA Library Construction and Use for Genetic (epmc).pdf DeepDyve Pubget Overpricing