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10.1016/j.jsb.2008.11.005 http://scihub22266oqcxt.onion/10.1016/j.jsb.2008.11.005 C4804765!4804765!19116171     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Struct+Biol 2009 ; 166 (1): 1-7 Nephropedia Template TP {{tp|p=19116171|t=2009. 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy |pdf=|usr=}}{{19116171}} gab.com Text 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy JO: J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=19116171 #FOAvip Understanding the hierarchical organization of molecules and organelles within the interior of large eukaryotic cells is a challenge of fundamental interest in cell biology. We are using ion-abrasion scanning electron microscopy (IA-SEM) to visualize this hierarchical organization in an approach that combines focused ion-beam milling with scanning electron microscopy. Here, we extend our previous studies on imaging yeast cells to image subcellular architecture in human melanoma cells and melanocytes at resolutions as high as ~6 and ~20 nm in the directions parallel and perpendicular, respectively, to the direction of ion-beam milling. The 3D images demonstrate the striking spatial relationships between specific organelles such as mitochondria and membranes of the endoplasmic reticulum, and the distribution of unique cellular components such as melanosomes. We also show that 10 nm-sized gold particles and quantum dot particles with 7 nm-sized cores can be detected in single cross-sectional images. IA-SEM is thus a useful tool for imaging large mammalian cells in their entirety at resolutions in the nanometer range. Twit Text FOAVip 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy ????J Struct Biol http://www.kidney.de/pget.php?&tw=1&pmid=19116171 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19116171 #FOAvip English Wikipedia <ref>{{Cite pmid|19116171}}</ref> 3D Imaging of mammalian cells with ion-abrasion scanning electron microscopy #MMPMID19116171 Heymann JA; Shi D; Kim S; Bliss D; Milne JL; Subramaniam S J Struct Biol 2009[Apr]; 166 (1): 1-7 PMID19116171show ga Understanding the hierarchical organization of molecules and organelles within the interior of large eukaryotic cells is a challenge of fundamental interest in cell biology. We are using ion-abrasion scanning electron microscopy (IA-SEM) to visualize this hierarchical organization in an approach that combines focused ion-beam milling with scanning electron microscopy. Here, we extend our previous studies on imaging yeast cells to image subcellular architecture in human melanoma cells and melanocytes at resolutions as high as ~6 and ~20 nm in the directions parallel and perpendicular, respectively, to the direction of ion-beam milling. The 3D images demonstrate the striking spatial relationships between specific organelles such as mitochondria and membranes of the endoplasmic reticulum, and the distribution of unique cellular components such as melanosomes. We also show that 10 nm-sized gold particles and quantum dot particles with 7 nm-sized cores can be detected in single cross-sectional images. IA-SEM is thus a useful tool for imaging large mammalian cells in their entirety at resolutions in the nanometer range. ä3D Imaging of mammalian cells with ion-abrasion scanning electron micr(epmc).pdf DeepDyve Pubget Overpricing