Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27004454
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
From morphology to biochemical state?-?intravital multiphoton fluorescence
lifetime imaging of inflamed human skin
#MMPMID27004454
Huck V
; Gorzelanny C
; Thomas K
; Getova V
; Niemeyer V
; Zens K
; Unnerstall TR
; Feger JS
; Fallah MA
; Metze D
; Ständer S
; Luger TA
; Koenig K
; Mess C
; Schneider SW
Sci Rep
2016[Mar]; 6
(?): 22789
PMID27004454
show ga
The application of multiphoton microscopy in the field of biomedical research and
advanced diagnostics promises unique insights into the pathophysiology of
inflammatory skin diseases. In the present study, we combined multiphoton-based
intravital tomography (MPT) and fluorescence lifetime imaging (MPT-FLIM) within
the scope of a clinical trial of atopic dermatitis with the aim of providing
personalised data on the aetiopathology of inflammation in a non-invasive manner
at patients' bedsides. These 'optical biopsies' generated via MPT were
morphologically analysed and aligned with classical skin histology. Because of
its subcellular resolution, MPT provided evidence of a redistribution of
mitochondria in keratinocytes, indicating an altered cellular metabolism. Two
independent morphometric algorithms reliably showed an even distribution in
healthy skin and a perinuclear accumulation in inflamed skin. Moreover, using
MPT-FLIM, detection of the onset and progression of inflammatory processes could
be achieved. In conclusion, the change in the distribution of mitochondria upon
inflammation and the verification of an altered cellular metabolism facilitate a
better understanding of inflammatory skin diseases and may permit early diagnosis
and therapy.
free
free
free
