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Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Bioinformatics 2016 ; 32 (6): 937-9 Nephropedia Template TP
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BioPartsBuilder: a synthetic biology tool for combinatorial assembly of biological parts #MMPMID26568632
Yang K; Stracquadanio G; Luo J; Boeke JD; Bader JS
Bioinformatics 2016[Mar]; 32 (6): 937-9 PMID26568632show ga
Summary: Combinatorial assembly of DNA elements is an efficient method for building large-scale synthetic pathways from standardized, reusable components. These methods are particularly useful because they enable assembly of multiple DNA fragments in one reaction, at the cost of requiring that each fragment satisfies design constraints. We developed BioPartsBuilder as a biologist-friendly web tool to design biological parts that are compatible with DNA combinatorial assembly methods, such as Golden Gate and related methods. It retrieves biological sequences, enforces compliance with assembly design standards and provides a fabrication plan for each fragment.Availability and implementation: BioPartsBuilder is accessible at http://public.biopartsbuilder.org and an Amazon Web Services image is available from the AWS Market Place (AMI ID: ami-508acf38). Source code is released under the MIT license, and available for download at https://github.com/baderzone/biopartsbuilder.Contact:joel.bader@jhu.eduSupplement ary information:Supplementary data are available at Bioinformatics online.