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10.3324/haematol.2014.114405 http://scihub22266oqcxt.onion/10.3324/haematol.2014.114405 C4803138!4803138 !25533803     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25533803 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Haematologica 2015 ; 100 (2 ): 178-87 Nephropedia Template TP {{tp|p=25533803 |t=2015. Dexamethasone targeted directly to macrophages induces macrophage niches that promote erythroid expansion |pdf=|usr=}}{{25533803 }} gab.com Text Dexamethasone targeted directly to macrophages induces macrophage niches that promote erythroid expansion JO: Haematologica http://www.kidney.de/pget.php?&tw=1&pmid=25533803 #FOAvip Cultures of human CD34(pos) cells stimulated with erythroid growth factors plus dexamethasone, a model for stress erythropoiesis, generate numerous erythroid cells plus a few macrophages (approx. 3%; 3:1 positive and negative for CD169). Interactions occurring between erythroblasts and macrophages in these cultures and the biological effects associated with these interactions were documented by live phase-contrast videomicroscopy. Macrophages expressed high motility interacting with hundreds/thousands of erythroblasts per hour. CD169(pos) macrophages established multiple rapid 'loose' interactions with proerythroblasts leading to formation of transient erythroblastic island-like structures. By contrast, CD169(neg) macrophages established 'tight' interactions with mature erythroblasts and phagocytosed these cells. 'Loose' interactions of CD169(pos) macrophages were associated with proerythroblast cytokinesis (the M phase of the cell cycle) suggesting that these interactions may promote proerythroblast duplication. This hypothesis was tested by experiments that showed that as few as 103 macrophages significantly increased levels of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide incorporation frequency in S/G2/M and cytokinesis expressed by proerythroblasts over 24 h of culture. These effects were observed also when macrophages were co-cultured with dexamethasone directly conjugated to a macrophage-specific CD163 antibody. In conclusion, in addition to promoting proerythroblast proliferation directly, dexamethasone stimulates expansion of these cells indirectly by stimulating maturation and cytokinesis supporting activity of macrophages. Twit Text FOAVip Dexamethasone targeted directly to macrophages induces macrophage niches that promote erythroid expansion ????Haematologica http://www.kidney.de/pget.php?&tw=1&pmid=25533803 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25533803 #FOAvip English Wikipedia <ref>{{Cite pmid|25533803 }}</ref> Dexamethasone targeted directly to macrophages induces macrophage niches that promote erythroid expansion #MMPMID25533803 Falchi M ; Varricchio L ; Martelli F ; Masiello F ; Federici G ; Zingariello M ; Girelli G ; Whitsett C ; Petricoin EF 3rd ; Moestrup SK ; Zeuner A ; Migliaccio AR Haematologica 2015[Feb]; 100 (2 ): 178-87 PMID25533803 show ga Cultures of human CD34(pos) cells stimulated with erythroid growth factors plus dexamethasone, a model for stress erythropoiesis, generate numerous erythroid cells plus a few macrophages (approx. 3%; 3:1 positive and negative for CD169). Interactions occurring between erythroblasts and macrophages in these cultures and the biological effects associated with these interactions were documented by live phase-contrast videomicroscopy. Macrophages expressed high motility interacting with hundreds/thousands of erythroblasts per hour. CD169(pos) macrophages established multiple rapid 'loose' interactions with proerythroblasts leading to formation of transient erythroblastic island-like structures. By contrast, CD169(neg) macrophages established 'tight' interactions with mature erythroblasts and phagocytosed these cells. 'Loose' interactions of CD169(pos) macrophages were associated with proerythroblast cytokinesis (the M phase of the cell cycle) suggesting that these interactions may promote proerythroblast duplication. This hypothesis was tested by experiments that showed that as few as 103 macrophages significantly increased levels of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide incorporation frequency in S/G2/M and cytokinesis expressed by proerythroblasts over 24 h of culture. These effects were observed also when macrophages were co-cultured with dexamethasone directly conjugated to a macrophage-specific CD163 antibody. In conclusion, in addition to promoting proerythroblast proliferation directly, dexamethasone stimulates expansion of these cells indirectly by stimulating maturation and cytokinesis supporting activity of macrophages. |Anti-Inflammatory Agents/*pharmacology [MESH] |Cell Differentiation/*drug effects [MESH] |Cell Proliferation/drug effects [MESH] |Cells, Cultured [MESH] |Coculture Techniques [MESH] |Cytokines/metabolism [MESH] |Dexamethasone/*pharmacology [MESH] |Erythroblasts/*cytology/drug effects/physiology [MESH] |Erythropoiesis/drug effects/*physiology [MESH] |Flow Cytometry [MESH] |Humans [MESH] |Macrophages/*cytology/drug effects/physiology [MESH] |Microscopy, Video [MESH]Dexamethasone targeted directly to macrophages induces macrophage nich(epmc).pdf DeepDyve Pubget Overpricing