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Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
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Effect of amygdalin on proliferation of rat hepatic fat-storing cells and collagen production in vitro #MMPMID27041958
Xu LM; Liu C; Liu P
World J Gastroenterol 1997[Jun]; 3 (2): 103 PMID27041958show ga
AIM: To study the anti-fibrotic mechanism of amygdalin, a component of Semen Persicae, on fat-storing cells (FSC).METHODS: Livers of normal adult rats were perfused with Pronas E and collagenase in situ. FSC were isolated by centrifugation with 11% Metrizamide. The subcultured FSC were incubated with 10-4-10-8 mol/L amygdalin for 72 h, and then FSC proliferation and collagen production were assayed, respectively.RESULTS: Low doses of amygdalin reduced incorporation of L-[3H]-thymidine into FSC and L-[5-3H]-proline into secreted collagenase-sensitive proteins and cell layer-associated collagenase-sensitive proteins. the strongest effects were seen for the 10-8 mol/L dose of amygdalin, which inhibited the proliferation of FSC by 25.0%, and decreased collagen production in medium and cell layer by 24.2% and 26.8%, respectively.CONCLUSION: An anti-fibrotic mechanism of amygdalin is to inhibit the proliferation and collagen production of active FSC.