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10.3748/wjg.v3.i2.103

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suck abstract from ncbi


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pmid27041958
      World+J+Gastroenterol 1997 ; 3 (2 ): 103
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  • Effect of amygdalin on proliferation of rat hepatic fat-storing cells and collagen production in vitro #MMPMID27041958
  • Xu LM ; Liu C ; Liu P
  • World J Gastroenterol 1997[Jun]; 3 (2 ): 103 PMID27041958 show ga
  • AIM: To study the anti-fibrotic mechanism of amygdalin, a component of Semen Persicae, on fat-storing cells (FSC). METHODS: Livers of normal adult rats were perfused with Pronas E and collagenase in situ. FSC were isolated by centrifugation with 11% Metrizamide. The subcultured FSC were incubated with 10(-4)-10(-8) mol/L amygdalin for 72 h, and then FSC proliferation and collagen production were assayed, respectively. RESULTS: Low doses of amygdalin reduced incorporation of L-[(3)H]-thymidine into FSC and L-[5-(3)H]-proline into secreted collagenase-sensitive proteins and cell layer-associated collagenase-sensitive proteins. the strongest effects were seen for the 10(-8) mol/L dose of amygdalin, which inhibited the proliferation of FSC by 25.0%, and decreased collagen production in medium and cell layer by 24.2% and 26.8%, respectively. CONCLUSION: An anti-fibrotic mechanism of amygdalin is to inhibit the proliferation and collagen production of active FSC.
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