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1997 ; 3
(2
): 103
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Effect of amygdalin on proliferation of rat hepatic fat-storing cells and
collagen production in vitro
#MMPMID27041958
Xu LM
; Liu C
; Liu P
World J Gastroenterol
1997[Jun]; 3
(2
): 103
PMID27041958
show ga
AIM: To study the anti-fibrotic mechanism of amygdalin, a component of Semen
Persicae, on fat-storing cells (FSC). METHODS: Livers of normal adult rats were
perfused with Pronas E and collagenase in situ. FSC were isolated by
centrifugation with 11% Metrizamide. The subcultured FSC were incubated with
10(-4)-10(-8) mol/L amygdalin for 72 h, and then FSC proliferation and collagen
production were assayed, respectively. RESULTS: Low doses of amygdalin reduced
incorporation of L-[(3)H]-thymidine into FSC and L-[5-(3)H]-proline into secreted
collagenase-sensitive proteins and cell layer-associated collagenase-sensitive
proteins. the strongest effects were seen for the 10(-8) mol/L dose of amygdalin,
which inhibited the proliferation of FSC by 25.0%, and decreased collagen
production in medium and cell layer by 24.2% and 26.8%, respectively. CONCLUSION:
An anti-fibrotic mechanism of amygdalin is to inhibit the proliferation and
collagen production of active FSC.