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10.1073/pnas.1601375113 http://scihub22266oqcxt.onion/10.1073/pnas.1601375113 C4801249!4801249 !26929379     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26929379 &cmd=llinks): Failed to open stream: HTTP request failed! 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Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway |pdf=|usr=}}{{26929379 }} gab.com Text Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26929379 #FOAvip Gram-negative bacteria balance synthesis of the outer membrane (OM), cell wall, and cytoplasmic contents during growth via unknown mechanisms. Here, we show that a dominant mutation (designated mlaA*, maintenance of lipid asymmetry) that alters MlaA, a lipoprotein that removes phospholipids from the outer leaflet of the OM of Escherichia coli, increases OM permeability, lipopolysaccharide levels, drug sensitivity, and cell death in stationary phase. Surprisingly, single-cell imaging revealed that death occurs after protracted loss of OM material through vesiculation and blebbing at cell-division sites and compensatory shrinkage of the inner membrane, eventually resulting in rupture and slow leakage of cytoplasmic contents. The death of mlaA* cells was linked to fatty acid depletion and was not affected by membrane depolarization, suggesting that lipids flow from the inner membrane to the OM in an energy-independent manner. Suppressor analysis suggested that the dominant mlaA* mutation activates phospholipase A, resulting in increased levels of lipopolysaccharide and OM vesiculation that ultimately undermine the integrity of the cell envelope by depleting the inner membrane of phospholipids. This novel cell-death pathway suggests that balanced synthesis across both membranes is key to the mechanical integrity of the Gram-negative cell envelope. Twit Text FOAVip Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=26929379 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26929379 #FOAvip English Wikipedia <ref>{{Cite pmid|26929379 }}</ref> Disruption of lipid homeostasis in the Gram-negative cell envelope activates a novel cell death pathway #MMPMID26929379 Sutterlin HA ; Shi H ; May KL ; Miguel A ; Khare S ; Huang KC ; Silhavy TJ Proc Natl Acad Sci U S A 2016[Mar]; 113 (11 ): E1565-74 PMID26929379 show ga Gram-negative bacteria balance synthesis of the outer membrane (OM), cell wall, and cytoplasmic contents during growth via unknown mechanisms. Here, we show that a dominant mutation (designated mlaA*, maintenance of lipid asymmetry) that alters MlaA, a lipoprotein that removes phospholipids from the outer leaflet of the OM of Escherichia coli, increases OM permeability, lipopolysaccharide levels, drug sensitivity, and cell death in stationary phase. Surprisingly, single-cell imaging revealed that death occurs after protracted loss of OM material through vesiculation and blebbing at cell-division sites and compensatory shrinkage of the inner membrane, eventually resulting in rupture and slow leakage of cytoplasmic contents. The death of mlaA* cells was linked to fatty acid depletion and was not affected by membrane depolarization, suggesting that lipids flow from the inner membrane to the OM in an energy-independent manner. Suppressor analysis suggested that the dominant mlaA* mutation activates phospholipase A, resulting in increased levels of lipopolysaccharide and OM vesiculation that ultimately undermine the integrity of the cell envelope by depleting the inner membrane of phospholipids. This novel cell-death pathway suggests that balanced synthesis across both membranes is key to the mechanical integrity of the Gram-negative cell envelope. |Bacterial Outer Membrane Proteins/genetics/metabolism [MESH] |Cell Membrane/chemistry/metabolism [MESH] |Cell Wall/*metabolism [MESH] |Escherichia coli Proteins/genetics/*metabolism [MESH] |Escherichia coli/cytology/drug effects/genetics/metabolism [MESH] |Fatty Acids/metabolism [MESH] |Lipid Metabolism/*genetics [MESH] |Lipopolysaccharides/metabolism [MESH] |Magnesium/metabolism/pharmacology [MESH] |Mutation [MESH] |Permeability [MESH] |Phospholipases A1/metabolism [MESH]Disruption of lipid homeostasis in the Gram-negative cell envelope act(epmc).pdf DeepDyve Pubget Overpricing