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10.1371/journal.pone.0151736 http://scihub22266oqcxt.onion/10.1371/journal.pone.0151736 C4801190!4801190!26998606     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2016 ; 11 (3): ä Nephropedia Template TP {{tp|p=26998606|t=2016. Extracellular Matrix Molecular Remodeling in Human Liver Fibrosis Evolution |pdf=|usr=}}{{26998606}} gab.com Text Extracellular Matrix Molecular Remodeling in Human Liver Fibrosis Evolution JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26998606 #FOAvip Chronic liver damage leads to pathological accumulation of ECM proteins (liver fibrosis). Comprehensive characterization of the human ECM molecular composition is essential for gaining insights into the mechanisms of liver disease. To date, studies of ECM remodeling in human liver diseases have been hampered by the unavailability of purified ECM. Here, we developed a decellularization method to purify ECM scaffolds from human liver tissues. Histological and electron microscopy analyses demonstrated that the ECM scaffolds, devoid of plasma and cellular components, preserved the three-dimensional ECM structure and zonal distribution of ECM components. This method has been then applied on 57 liver biopsies of HCV-infected patients at different stages of liver fibrosis according to METAVIR classification. Label-free nLC-MS/MS proteomics and computation biology were performed to analyze the ECM molecular composition in liver fibrosis progression, thus unveiling protein expression signatures specific for the HCV-related liver fibrotic stages. In particular, the ECM molecular composition of liver fibrosis was found to involve dynamic changes in matrix stiffness, flexibility and density related to the dysregulation of predominant collagen, elastic fibers and minor components with both structural and signaling properties. This study contributes to the understanding of the molecular bases underlying ECM remodeling in liver fibrosis and suggests new molecular targets for fibrolytic strategies. Twit Text FOAVip Extracellular Matrix Molecular Remodeling in Human Liver Fibrosis Evolution ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=26998606 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26998606 #FOAvip English Wikipedia <ref>{{Cite pmid|26998606}}</ref> Extracellular Matrix Molecular Remodeling in Human Liver Fibrosis Evolution #MMPMID26998606 Baiocchini A; Montaldo C; Conigliaro A; Grimaldi A; Correani V; Mura F; Ciccosanti F; Rotiroti N; Brenna A; Montalbano M; D?Offizi G; Capobianchi MR; Alessandro R; Piacentini M; Schininà ME; Maras B; Del Nonno F; Tripodi M; Mancone C PLoS One 2016[]; 11 (3): ä PMID26998606show ga Chronic liver damage leads to pathological accumulation of ECM proteins (liver fibrosis). Comprehensive characterization of the human ECM molecular composition is essential for gaining insights into the mechanisms of liver disease. To date, studies of ECM remodeling in human liver diseases have been hampered by the unavailability of purified ECM. Here, we developed a decellularization method to purify ECM scaffolds from human liver tissues. Histological and electron microscopy analyses demonstrated that the ECM scaffolds, devoid of plasma and cellular components, preserved the three-dimensional ECM structure and zonal distribution of ECM components. This method has been then applied on 57 liver biopsies of HCV-infected patients at different stages of liver fibrosis according to METAVIR classification. Label-free nLC-MS/MS proteomics and computation biology were performed to analyze the ECM molecular composition in liver fibrosis progression, thus unveiling protein expression signatures specific for the HCV-related liver fibrotic stages. In particular, the ECM molecular composition of liver fibrosis was found to involve dynamic changes in matrix stiffness, flexibility and density related to the dysregulation of predominant collagen, elastic fibers and minor components with both structural and signaling properties. This study contributes to the understanding of the molecular bases underlying ECM remodeling in liver fibrosis and suggests new molecular targets for fibrolytic strategies. äExtracellular Matrix Molecular Remodeling in Human Liver Fibrosis Evol(epmc).pdf DeepDyve Pubget Overpricing