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Small airway epithelial cells exposure to printer-emitted engineered
nanoparticles induces cellular effects on human microvascular endothelial cells
in an alveolar-capillary co-culture model
#MMPMID25387250
Sisler JD
; Pirela SV
; Friend S
; Farcas M
; Schwegler-Berry D
; Shvedova A
; Castranova V
; Demokritou P
; Qian Y
Nanotoxicology
2015[]; 9
(6
): 769-79
PMID25387250
show ga
The printer is one of the most common office equipment. Recently, it was reported
that toner formulations for printing equipment constitute nano-enabled products
(NEPs) and contain engineered nanomaterials (ENMs) that become airborne during
printing. To date, insufficient research has been performed to understand the
potential toxicological properties of printer-emitted particles (PEPs) with
several studies using bulk toner particles as test particles. These studies
demonstrated the ability of toner particles to cause chronic inflammation and
fibrosis in animal models. However, the toxicological implications of inhalation
exposures to ENMs emitted from laser printing equipment remain largely unknown.
The present study investigates the toxicological effects of PEPs using an in
vitro alveolar-capillary co-culture model with Human Small Airway Epithelial
Cells (SAEC) and Human Microvascular Endothelial Cells (HMVEC). Our data
demonstrate that direct exposure of SAEC to low concentrations of PEPs (0.5 and
1.0?µg/mL) caused morphological changes of actin remodeling and gap formations
within the endothelial monolayer. Furthermore, increased production of reactive
oxygen species (ROS) and angiogenesis were observed in the HMVEC. Analysis of
cytokine and chemokine levels demonstrates that interleukin (IL)-6 and MCP-1 may
play a major role in the cellular communication observed between SAEC and HMVEC
and the resultant responses in HMVEC. These data indicate that PEPs at low,
non-cytotoxic exposure levels are bioactive and affect cellular responses in an
alveolar-capillary co-culture model, which raises concerns for potential adverse
health effects.
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