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2016 ; 2016
(ä): 4608979
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Protective Role of Ramipril and Candesartan against Myocardial Ischemic
Reperfusion Injury: A Biochemical and Transmission Electron Microscopical Study
#MMPMID27042175
Sheik Uduman MS
; Reddy RB
; Punuru P
; Chakka G
; Karunakaran G
Adv Pharmacol Sci
2016[]; 2016
(ä): 4608979
PMID27042175
show ga
The present study was designed to investigate the role of combined administration
of Ramipril and Candesartan against in vitro myocardial ischemic reperfusion
injury in rat. Male Wistar albino rats were divided into five groups (n = 6) and
treated with saline (10?mL/kg), Ramipril (2?mg/kg), Candesartan (1?mg/kg), and
the combination of both drugs, respectively 24?h before induction of global
ischemia (5?min of stabilization, 9?min of global ischemia, and 12?min of
reflow). Combination of Ramipril and Candesartan when compared to the monotherapy
significantly increased the levels of superoxide dismutase, reduced glutathione,
catalase, and nitric oxide and decreased the levels of thiobarbituric acid
reactive substances. In addition, the superior protective role of combination of
Ramipril and Candesartan on ischemia induced myocardial damage was further
confirmed by well preserved myocardial tissue architecture in light microscopy
and transmission electron microscopy analysis studies. The combination was proved
to be effective in salvaging the myocardial tissue against ischemic reperfusion
injury when compared to the monotherapy of individual drugs and further
investigations on protective mechanism of drugs by increasing the nitric oxide
level at molecular levels are needed.