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10.1093/neuonc/nov170 http://scihub22266oqcxt.onion/10.1093/neuonc/nov170 C4799676!4799676!26385614     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Neuro+Oncol 2016 ; 18 (4): 497-506 Nephropedia Template TP {{tp|p=26385614|t=2016. Glioma-derived extracellular vesicles selectively suppress immune responses |pdf=|usr=}}{{26385614}} gab.com Text Glioma-derived extracellular vesicles selectively suppress immune responses JO: Neuro Oncol http://www.kidney.de/pget.php?&tw=1&pmid=26385614 #FOAvip Background: Glioma-related immunosuppression is well documented; however, the mechanisms of suppression are not fully understood. Here we explore a role for glioma extracellular vesicles (EVs) as a means of immune modulation. Methods: Healthy donor peripheral blood mononuclear cells (PBMCs) were incubated with mitogenic stimuli and various concentrations of glioma-derived EVs. Intracellular signaling and cytokine output were determined by protein microarrays, and phenotypic changes were assessed by flow cytometry. Recall antigen testing, mixed lymphocyte reactions, and migration assays analyzed PBMC functional capacity. Results: Protein microarray data revealed induction of an immunosuppressive phenotype and cytokine output at high tumor-vesicle concentrations but an activated phenotype at low concentrations. T cell activation antigen expression confirmed differential activation profiles. Functional analyses revealed decreased migratory capacity of PBMCs after incubation with EVs; however, recall antigen and mixed lymphocyte tests indicated that activation capacity is still retained in EV-treated cells. Conclusion: The differential effects of high and low EV concentrations dictate modulatory effects on PBMCs. These data provide a role for EVs at high concentrations for inducing selective tolerance of an immune response in a tumor setting. This suggests that lymphocytes in patients? circulation are not irreparably impaired, as previously thought, but can be rescued to augment antitumor responses. Twit Text FOAVip Glioma-derived extracellular vesicles selectively suppress immune responses ????Neuro Oncol http://www.kidney.de/pget.php?&tw=1&pmid=26385614 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26385614 #FOAvip English Wikipedia <ref>{{Cite pmid|26385614}}</ref> Glioma-derived extracellular vesicles selectively suppress immune responses #MMPMID26385614 Hellwinkel JE; Redzic JS; Harland TA; Gunaydin D; Anchordoquy TJ; Graner MW Neuro Oncol 2016[Apr]; 18 (4): 497-506 PMID26385614show ga Background: Glioma-related immunosuppression is well documented; however, the mechanisms of suppression are not fully understood. Here we explore a role for glioma extracellular vesicles (EVs) as a means of immune modulation. Methods: Healthy donor peripheral blood mononuclear cells (PBMCs) were incubated with mitogenic stimuli and various concentrations of glioma-derived EVs. Intracellular signaling and cytokine output were determined by protein microarrays, and phenotypic changes were assessed by flow cytometry. Recall antigen testing, mixed lymphocyte reactions, and migration assays analyzed PBMC functional capacity. Results: Protein microarray data revealed induction of an immunosuppressive phenotype and cytokine output at high tumor-vesicle concentrations but an activated phenotype at low concentrations. T cell activation antigen expression confirmed differential activation profiles. Functional analyses revealed decreased migratory capacity of PBMCs after incubation with EVs; however, recall antigen and mixed lymphocyte tests indicated that activation capacity is still retained in EV-treated cells. Conclusion: The differential effects of high and low EV concentrations dictate modulatory effects on PBMCs. These data provide a role for EVs at high concentrations for inducing selective tolerance of an immune response in a tumor setting. This suggests that lymphocytes in patients? circulation are not irreparably impaired, as previously thought, but can be rescued to augment antitumor responses. äGlioma-derived extracellular vesicles selectively suppress immune resp(epmc).pdf DeepDyve Pubget Overpricing