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10.1093/infdis/jiv588 http://scihub22266oqcxt.onion/10.1093/infdis/jiv588 C4799669!4799669!26656125     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Infect+Dis 2016 ; 213 (8): 1307-14 Nephropedia Template TP {{tp|p=26656125|t=2016. Preassembled Single-Stranded RNA?Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium |pdf=|usr=}}{{26656125}} gab.com Text Preassembled Single-Stranded RNA Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium JO: J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=26656125 #FOAvip Cryptosporidiosis is a common cause of diarrhea morbidity and mortality worldwide. Research progress on this infection has been slowed by lack of methods to genetically manipulate Cryptosporidium parasites. Small interfering RNA (siRNA) is widely used to study gene function, but Cryptosporidium species lack the enzymes necessary to process siRNA. By preassembling complexes with the human enzyme Argonaute 2 (hAgo2) and Cryptosporidium single-stranded RNA (ssRNA), we induced specific slicing in Cryptosporidium RNA targets. We demonstrated the reduction in expression of target genes at the mRNA and protein levels by transfecting live parasites with ssRNA-hAgo2 complexes. Furthermore we used this method to confirm the role of selected molecules during host cell invasion. This novel method provides a novel means of silencing Cryptosporidium genes to study their role in host-parasite interactions and as potential targets for chemotherapy. Twit Text FOAVip Preassembled Single-Stranded RNA Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium ????J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=26656125 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26656125 #FOAvip English Wikipedia <ref>{{Cite pmid|26656125}}</ref> Preassembled Single-Stranded RNA?Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium #MMPMID26656125 Castellanos-Gonzalez A; Perry N; Nava S; White AC J Infect Dis 2016[Apr]; 213 (8): 1307-14 PMID26656125show ga Cryptosporidiosis is a common cause of diarrhea morbidity and mortality worldwide. Research progress on this infection has been slowed by lack of methods to genetically manipulate Cryptosporidium parasites. Small interfering RNA (siRNA) is widely used to study gene function, but Cryptosporidium species lack the enzymes necessary to process siRNA. By preassembling complexes with the human enzyme Argonaute 2 (hAgo2) and Cryptosporidium single-stranded RNA (ssRNA), we induced specific slicing in Cryptosporidium RNA targets. We demonstrated the reduction in expression of target genes at the mRNA and protein levels by transfecting live parasites with ssRNA-hAgo2 complexes. Furthermore we used this method to confirm the role of selected molecules during host cell invasion. This novel method provides a novel means of silencing Cryptosporidium genes to study their role in host-parasite interactions and as potential targets for chemotherapy. äPreassembled Single-Stranded RNA?Argonaute Complexes: A Novel Method t(epmc).pdf DeepDyve Pubget Overpricing