Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1101/pdb.top067918 http://scihub22266oqcxt.onion/10.1101/pdb.top067918 C4799658!4799658!22301660     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cold+Spring+Harb+Protoc 2012 ; 2012 (2): 141-9 Nephropedia Template TP {{tp|p=22301660|t=2012. Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution |pdf=|usr=}}{{22301660}} gab.com Text Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution JO: Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22301660 #FOAvip Colocalization of fluorescent probes is commonly used in cell biology to discern the proximity of two proteins in the cell. Considering that the resolution limit of optical microscopy is on the order of 250 nm, there has not been a need for high-resolution colocalization techniques. However, with the advent of higher resolution techniques for cell biology and single-molecule biophysics, colocalization must also improve. For diffraction-limited applications, a geometric transformation (i.e., translation, scaling, and rotation) is typically applied to one color channel to align it with the other; however, to achieve high-resolution colocalization, this is not sufficient. Single-molecule high-resolution colocalization (SHREC) of single probes uses the local weighted mean transformation to achieve a colocalization resolution of at least 10 nm. This article describes the process of collecting a calibration data set of fiducials and the appropriate analysis to determine the transformation for colocalization. Twit Text FOAVip Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution ????Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22301660 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22301660 #FOAvip English Wikipedia <ref>{{Cite pmid|22301660}}</ref> Colocalization of Fluorescent Probes: Accurate and Precise Registration with Nanometer Resolution #MMPMID22301660 Churchman LS; Spudich JA Cold Spring Harb Protoc 2012[Feb]; 2012 (2): 141-9 PMID22301660show ga Colocalization of fluorescent probes is commonly used in cell biology to discern the proximity of two proteins in the cell. Considering that the resolution limit of optical microscopy is on the order of 250 nm, there has not been a need for high-resolution colocalization techniques. However, with the advent of higher resolution techniques for cell biology and single-molecule biophysics, colocalization must also improve. For diffraction-limited applications, a geometric transformation (i.e., translation, scaling, and rotation) is typically applied to one color channel to align it with the other; however, to achieve high-resolution colocalization, this is not sufficient. Single-molecule high-resolution colocalization (SHREC) of single probes uses the local weighted mean transformation to achieve a colocalization resolution of at least 10 nm. This article describes the process of collecting a calibration data set of fiducials and the appropriate analysis to determine the transformation for colocalization. äColocalization of Fluorescent Probes: Accurate and Precise Registratio(epmc).pdf DeepDyve Pubget Overpricing