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10.1101/pdb.prot066977 http://scihub22266oqcxt.onion/10.1101/pdb.prot066977 C4799655!4799655!22135665     free     free     free       Cold+Spring+Harb+Protoc 2011 ; 2011 (12): 1498-506 Nephropedia Template TP {{tp|p=22135665|t=2011. Single-Molecule Gold-Nanoparticle Tracking |pdf=|usr=}}{{22135665}} gab.com Text Single-Molecule Gold-Nanoparticle Tracking JO: Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22135665 #FOAvip Gold nanoparticles, like single fluorophores, can be used to locate single molecules with nanometer accuracy. Unlike an optical trap, the gold particle label does not exert an external load, which is important for studying diffusive processes. Thus, a gold particle can be used analogously to a single fluorophore, providing similar information but with submillisecond time resolution. The features of gold-nanoparticle tracking (high temporal resolution, small label size, and lack of applied force) facilitate the characterization of structural properties of short-lived intermediates, as shown by our work with myosin V. This protocol provides details for gold-nanoparticle-tracking experiments, including flow cell construction, microscopy, and data analysis, along with a brief outline of actin and myosin preparation. Although details particular to our experiment are given, the approach should be generally applicable. Twit Text FOAVip Single-Molecule Gold-Nanoparticle Tracking ????Cold Spring Harb Protoc http://www.kidney.de/pget.php?&tw=1&pmid=22135665 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22135665 #FOAvip English Wikipedia <ref>{{Cite pmid|22135665}}</ref> Single-Molecule Gold-Nanoparticle Tracking #MMPMID22135665 Dunn AR; Spudich JA Cold Spring Harb Protoc 2011[Dec]; 2011 (12): 1498-506 PMID22135665show ga Gold nanoparticles, like single fluorophores, can be used to locate single molecules with nanometer accuracy. Unlike an optical trap, the gold particle label does not exert an external load, which is important for studying diffusive processes. Thus, a gold particle can be used analogously to a single fluorophore, providing similar information but with submillisecond time resolution. The features of gold-nanoparticle tracking (high temporal resolution, small label size, and lack of applied force) facilitate the characterization of structural properties of short-lived intermediates, as shown by our work with myosin V. This protocol provides details for gold-nanoparticle-tracking experiments, including flow cell construction, microscopy, and data analysis, along with a brief outline of actin and myosin preparation. Although details particular to our experiment are given, the approach should be generally applicable. äSingle-Molecule Gold-Nanoparticle Tracking (epmc).pdf DeepDyve Pubget Overpricing