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Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 F1000Res 2015 ; 4 (ä): ä Nephropedia Template TP
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Metagenomic Chromosome Conformation Capture (3C): techniques, applications, and challenges #MMPMID27006753
Liu M; Darling A
F1000Res 2015[]; 4 (ä): ä PMID27006753show ga
We review currently available technologies for deconvoluting metagenomic data into individual genomes that represent populations, strains, or genotypes present in the community. An evaluation of chromosome conformation capture (3C) and related techniques in the context of metagenomics is presented, using mock microbial communities as a reference. We provide the first independent reproduction of the metagenomic 3C technique described last year, propose some simple improvements to that protocol, and compare the quality of the data with that provided by the more complex Hi-C protocol.