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10.4137/BMI.S26229

http://scihub22266oqcxt.onion/10.4137/BMI.S26229
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C4795486!4795486!26997865
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suck abstract from ncbi


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pmid26997865      Biomark+Insights 2016 ; 11 (ä): 41-8
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  • Urine Proteome Biomarkers in Kidney Diseases I Limits, Perspectives, and First Focus on Normal Urine #MMPMID26997865
  • Santucci L; Bruschi M; Candiano G; Lugani F; Petretto A; Bonanni A; Ghiggeri GM
  • Biomark Insights 2016[]; 11 (ä): 41-8 PMID26997865show ga
  • Urine proteome is a potential source of information in renal diseases, and it is considered a natural area of investigation for biomarkers. Technology developments have markedly increased the power analysis on urinary proteins, and it is time to confront methodologies and results of major studies on the topics. This is a first part of a series of reviews that will focus on the urine proteome as a site for detecting biomarkers of renal diseases; the theme of the first review concerns methodological aspects applied to normal urine. Main issues are techniques for urine pretreatment, separation of exosomes, use of combinatorial peptide ligand libraries, mass spectrometry approaches, and analysis of data sets. Available studies show important differences, suggesting a major confounding effect of the technologies utilized for analysis. The objective is to obtain consensus about which approaches should be utilized for studying urine proteome in renal diseases.
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