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2016 ; 11
(ä): 41-8
Nephropedia Template TP
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Urine Proteome Biomarkers in Kidney Diseases I Limits, Perspectives, and First
Focus on Normal Urine
#MMPMID26997865
Santucci L
; Bruschi M
; Candiano G
; Lugani F
; Petretto A
; Bonanni A
; Ghiggeri GM
Biomark Insights
2016[]; 11
(ä): 41-8
PMID26997865
show ga
Urine proteome is a potential source of information in renal diseases, and it is
considered a natural area of investigation for biomarkers. Technology
developments have markedly increased the power analysis on urinary proteins, and
it is time to confront methodologies and results of major studies on the topics.
This is a first part of a series of reviews that will focus on the urine proteome
as a site for detecting biomarkers of renal diseases; the theme of the first
review concerns methodological aspects applied to normal urine. Main issues are
techniques for urine pretreatment, separation of exosomes, use of combinatorial
peptide ligand libraries, mass spectrometry approaches, and analysis of data
sets. Available studies show important differences, suggesting a major
confounding effect of the technologies utilized for analysis. The objective is to
obtain consensus about which approaches should be utilized for studying urine
proteome in renal diseases.