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10.1007/978-1-61779-163-5_4

http://scihub22266oqcxt.onion/10.1007/978-1-61779-163-5_4
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C4794997!4794997!21761293
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suck abstract from ncbi

pmid21761293      Methods+Mol+Biol 2011 ; 755 (ä): 57-66
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  • Immunoguided Microdissection Techniques #MMPMID21761293
  • Tangrea MA; Hanson JC; Bonner RF; Pohida TJ; Rodriguez-Canales J; Emmert-Buck MR
  • Methods Mol Biol 2011[]; 755 (ä): 57-66 PMID21761293show ga
  • Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems.
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