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10.1111/mmi.13294 http://scihub22266oqcxt.onion/10.1111/mmi.13294 C4794775!4794775!26714274     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Microbiol 2016 ; 99 (6): 1119-33 Nephropedia Template TP {{tp|p=26714274|t=2016. Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes |pdf=|usr=}}{{26714274}} gab.com Text Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes JO: Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26714274 #FOAvip Group A Streptococcus (GAS) is an exclusive human pathogen that causes significant disease burden. Global regulator RopB of GAS controls the expression of several major virulence factors including secreted protease SpeB during high cell density. However, the molecular mechanism for RopB?dependent speB expression remains unclear. To understand the mechanism of transcription activation by RopB, we determined the crystal structure of the C?terminal domain of RopB. RopB?CTD has the TPR motif, a signature motif involved in protein?peptide interactions and shares significant structural homology with the quorum sensing RRNPP family regulators. Characterization of the high cell density?specific cell?free growth medium demonstrated the presence of a low molecular weight proteinaceous secreted factor that upregulates RopB?dependent speB expression. Together, these results suggest that RopB and its cognate peptide signals constitute an intercellular signalling machinery that controls the virulence gene expression in concert with population density. Structure?guided mutational analyses of RopB dimer interface demonstrated that single alanine substitutions at this critical interface significantly altered RopB?dependent speB expression and attenuated GAS virulence. Results presented here suggested that a properly aligned RopB dimer interface is important for GAS pathogenesis and highlighted the dimerization interactions as a plausible therapeutic target for the development of novel antimicrobials. Twit Text FOAVip Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes ????Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26714274 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26714274 #FOAvip English Wikipedia <ref>{{Cite pmid|26714274}}</ref> Structural and functional analysis of RopB: a major virulence regulator in Streptococcus pyogenes #MMPMID26714274 Makthal N; Gavagan M; Do H; Olsen RJ; Musser JM; Kumaraswami M Mol Microbiol 2016[Mar]; 99 (6): 1119-33 PMID26714274show ga Group A Streptococcus (GAS) is an exclusive human pathogen that causes significant disease burden. Global regulator RopB of GAS controls the expression of several major virulence factors including secreted protease SpeB during high cell density. However, the molecular mechanism for RopB?dependent speB expression remains unclear. To understand the mechanism of transcription activation by RopB, we determined the crystal structure of the C?terminal domain of RopB. RopB?CTD has the TPR motif, a signature motif involved in protein?peptide interactions and shares significant structural homology with the quorum sensing RRNPP family regulators. Characterization of the high cell density?specific cell?free growth medium demonstrated the presence of a low molecular weight proteinaceous secreted factor that upregulates RopB?dependent speB expression. Together, these results suggest that RopB and its cognate peptide signals constitute an intercellular signalling machinery that controls the virulence gene expression in concert with population density. Structure?guided mutational analyses of RopB dimer interface demonstrated that single alanine substitutions at this critical interface significantly altered RopB?dependent speB expression and attenuated GAS virulence. Results presented here suggested that a properly aligned RopB dimer interface is important for GAS pathogenesis and highlighted the dimerization interactions as a plausible therapeutic target for the development of novel antimicrobials. äStructural and functional analysis of RopB: a major virulence regulato(epmc).pdf DeepDyve Pubget Overpricing