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Sialyltransferase and Neuraminidase Levels/Ratios and Sialic Acid Levels in
Peripheral Blood B Cells Correlate with Measures of Disease Activity in Patients
with Systemic Lupus Erythematosus and Rheumatoid Arthritis: A Pilot Study
#MMPMID26981635
Liou LB
; Huang CC
PLoS One
2016[]; 11
(3
): e0151669
PMID26981635
show ga
OBJECTIVE: We attempted to determine whether the level of enzymes
sialyltransferase (ST) and neuraminidase (Neu) and sialic acid (SIA) in patients
with systemic lupus erythematosus (SLE) correlates with the SLE Disease Activity
Index (SLEDAI) and in patients with rheumatoid arthritis (RA) correlates with the
Disease Activity Score28 (DAS28). METHODS: We examined cell-surface levels of
ST6Gal-1, Neu1, ST3Gal-1, Neu3, ?-2,6-SIA, and ?-2,3-SIA by using fluorescent
anti-enzyme antibodies, fluorescent-conjugated Sambucus nigra lectin, and
fluorescent-conjugated Maackia amurensis lectin on blood cells in SLE and RA
patients and assessed correlations of these levels with SLEDAI and with DAS28.
Areas under the curve (AUC) were calculated for different variables against
SLEDAI. RESULTS: The B-cell ST3Gal-1/Neu3 ratio positively correlated with SLEDAI
scores (? = 0.409 and P = 0.002, statistically significant after Bonferroni'
correction for multiple analyses.). It was supported by the inverse correlation
of B-cell Neu3 levels with SLEDAI scores (? = -0.264, P = 0.048). The B-cell
ST3Gal-1/Neu3 ratio against SLEDAI yielded an AUC of 0.689, which was comparable
to that of anti-dsDNA levels at 0.635. In contrast, both ST3Gal-1 and Neu3 levels
of RA B cells (r = 0.376, P = 0.013; r = 0.425, P = 0.005, respectively)
correlated positively with high disease-activity DAS28 scores. CONCLUSION: B-cell
ST3Gal-1/Neu3 ratios in SLE and B-cell ST3Gal-1 and Neu3 levels in RA with high
disease-activity DAS28 scores correlated with disease activity measures and may
be useful in monitoring disease activities.
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