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10.1186/s13742-016-0119-4

http://scihub22266oqcxt.onion/10.1186/s13742-016-0119-4
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suck abstract from ncbi


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pmid26985364      Gigascience 2016 ; 5 (ä): ä
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  • Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes) #MMPMID26985364
  • Carbayo F; Lenihan JW
  • Gigascience 2016[]; 5 (ä): ä PMID26985364show ga
  • Background: We investigated whether images obtained through X-ray micro-computed tomography (?CT) can be used in conjunction with traditional methods for morphological studies of soft-bodied land planarians. ?CT is non-invasive and provides true-to-scale three-dimensional imagery at high resolution. We compared ?CT-based images of a recently described land planarian species of Obama otavioi (Platyhelminthes) with those obtained from light microphotography of histological sections, most of which were also digitized at high magnification. Findings: The specimens studied were collected in 2012. Subsequent ?CT-based images of the stained body of a paratype show nearly all morphological features provided by traditional histology, with the exception of particularly minute structures, smaller than 5 ?m, such as the sensory pits and single muscle fibers, which are best visible on traditional histological sections. Because the technique is non-destructive, the scanned specimen is preserved without damage. The raw and derivative ?CT data and virtual histological sections are freely available in GigaDB. Conclusions: The ?CT datasets of these stained soft-bodied organisms reveal images of external and internal structures that support previous taxonomic studies. This technique can be particularly important for non-destructively revealing internal details of whole museum specimens at a faster rate than histology alone. High-resolution virtual histological slides also allow further searches for new, previously unstudied morphological features. The use of X-ray equipment with higher resolution can enable smaller sensory organ and muscle fiber details to be seen. The image sets, ?CT-based images and digitized histological slides can be disseminated without the constraints of specimen loans.
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