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10.1186/s13071-016-1415-7

http://scihub22266oqcxt.onion/10.1186/s13071-016-1415-7
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suck abstract from ncbi


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pmid26983858      Parasit+Vectors 2016 ; 9 (ä): ä
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  • Horizontal transfer of ?-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes #MMPMID26983858
  • Zolfaghari Emameh R; Barker HR; Tolvanen MEE; Parkkila S; Hytönen VP
  • Parasit Vectors 2016[]; 9 (ä): ä PMID26983858show ga
  • Background: Horizontal gene transfer (HGT) is a movement of genetic information occurring outside of normal mating activities. It is especially common between prokaryotic endosymbionts and their protozoan, insect, and nematode hosts. Although beta carbonic anhydrase (?-CA) plays a crucial role in metabolic functions of many living organisms, the origin of ?-CA genes in eukaryotic species remains unclear. Methods: This study was conducted using phylogenetics, prediction of subcellular localization, and identification of ?-CA, transposase, integrase, and resolvase genes on the MGEs of bacteria. We also structurally analyzed ?-CAs from protozoans, insects, and nematodes and their putative prokaryotic common ancestors, by homology modelling. Results: Our investigations of a number of target genomes revealed that genes coding for transposase, integrase, resolvase, and conjugation complex proteins have been integrated with ?-CA gene sequences on mobile genetic elements (MGEs) which have facilitated the mobility of ?-CA genes from bacteria to protozoan, insect, and nematode species. The prokaryotic origin of protozoan, insect, and nematode ?-CA enzymes is supported by phylogenetic analyses, prediction of subcellular localization, and homology modelling. Conclusion: MGEs form a complete set of enzymatic tools, which are relevant to HGT of ?-CA gene sequences from prokaryotes to protozoans, insects, and nematodes. Electronic supplementary material: The online version of this article (doi:10.1186/s13071-016-1415-7) contains supplementary material, which is available to authorized users.
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