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10.1038/ncomms10945 http://scihub22266oqcxt.onion/10.1038/ncomms10945 C4792949!4792949!26961578     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26961578&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26961578|t=2016. Damage-free vibrational spectroscopy of biological materials in the electron microscope |pdf=|usr=}}{{26961578}} gab.com Text Damage-free vibrational spectroscopy of biological materials in the electron microscope JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26961578 #FOAvip Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ?aloof' electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies <1?eV can be ?safely' investigated. To demonstrate the potential of aloof spectroscopy, we record electron energy loss spectra from biogenic guanine crystals in their native state, resolving their characteristic C?H, N?H and C=O vibrational signatures with no observable radiation damage. The technique opens up the possibility of non-damaging compositional analyses of organic functional groups, including non-crystalline biological materials, at a spatial resolution of ?10?nm, simultaneously combined with imaging in the electron microscope. Twit Text FOAVip Damage-free vibrational spectroscopy of biological materials in the electron microscope ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26961578 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26961578 #FOAvip English Wikipedia <ref>{{Cite pmid|26961578}}</ref> Damage-free vibrational spectroscopy of biological materials in the electron microscope #MMPMID26961578 Rez P; Aoki T; March K; Gur D; Krivanek OL; Dellby N; Lovejoy TC; Wolf SG; Cohen H Nat Commun 2016[]; 7 (ä): ä PMID26961578show ga Vibrational spectroscopy in the electron microscope would be transformative in the study of biological samples, provided that radiation damage could be prevented. However, electron beams typically create high-energy excitations that severely accelerate sample degradation. Here this major difficulty is overcome using an ?aloof' electron beam, positioned tens of nanometres away from the sample: high-energy excitations are suppressed, while vibrational modes of energies <1?eV can be ?safely' investigated. To demonstrate the potential of aloof spectroscopy, we record electron energy loss spectra from biogenic guanine crystals in their native state, resolving their characteristic C?H, N?H and C=O vibrational signatures with no observable radiation damage. The technique opens up the possibility of non-damaging compositional analyses of organic functional groups, including non-crystalline biological materials, at a spatial resolution of ?10?nm, simultaneously combined with imaging in the electron microscope. äDamage-free vibrational spectroscopy of biological materials in the el(epmc).pdf DeepDyve Pubget Overpricing