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Astragalus polysaccharide attenuates rat experimental colitis by inducing
regulatory T cells in intestinal Peyer s patches
#MMPMID27003994
Zhao HM
; Wang Y
; Huang XY
; Huang MF
; Xu R
; Yue HY
; Zhou BG
; Huang HY
; Sun QM
; Liu DY
World J Gastroenterol
2016[Mar]; 22
(11
): 3175-85
PMID27003994
show ga
AIM: To explore probable mechanism underlying the therapeutic effect of
Astragalus polysaccharide (APS) against experimental colitis. METHODS: Thirty-two
Sprague-Dawley rats were randomly divided into four groups. Colitis was induced
with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). The rats with colitis were
treated with 400 mg/kg of APS for 7 d. The therapeutic effect was evaluated by
colonic weight, weight index of the colon, colonic length, and macroscopic and
histological scores. The levels of regulatory T (Treg) cells in Peyer's patches
were measured by flow cytometry, and cytokines in colonic tissue homogenates were
analyzed using enzyme-linked immunosorbent assay. The expression of related
orphan receptor-?t (ROR-?t), IL-23 and STAT-5a was measured by Western blot.
RESULTS: After 7-d treatment with APS, the weight index of the colon, colonic
weight, macroscopical and histological scores were decreased, while the colonic
length was increased compared with the model group. The expression of interleukin
(IL)-2, IL-6, IL-17, IL-23 and ROR-?t in the colonic tissues was down-regulated,
but Treg cells in Peyer's patches, TGF-? and STAT5a in the colonic tissues were
up-regulated. CONCLUSION: APS effectively ameliorates TNBS-induced experimental
colitis in rats, probably through restoring the number of Treg cells, and
inhibiting IL-17 levels in Peyer's patches.
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