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2010 ; 1
(3
): 115-22
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Autografting of Renal Progenitor Cells Ameliorates Kidney Damage in Experimental
Model of Pyelonephritis
#MMPMID27004131
Kajbafzadeh AM
; Elmi A
; Talab SS
; Sadeghi Z
; Emami H
; Sotoudeh M
Cell Med
2010[]; 1
(3
): 115-22
PMID27004131
show ga
Current therapies for pyelonephritic renal damage have severe limitations; stem
cells may offer an exciting potential in regenerating nephrology. We aimed to
investigate the feasibility of direct intrarenal injection of autologous renal
progenitor cells (RPCs; originated from epithelial cells in Bowman's capsule) in
chronic pyelonephritis rat model. Twenty-seven rats were divided into three
groups. The control group (GI, n?=?3) underwent sham subcapsular injection of
isotonic saline. Pyelonephritis was induced in the right kidney of the remaining
24 rats and isotonic saline (GII, n?=?12) or labeled autologous RPCs, obtained
from a biopsy of left kidney (GIII, n?=?12), were injected into the subcapsular
space 6 weeks later. At 7, 14, 28, and 60 days, dimercaptosuccinic acid scan was
performed in three animals of each group at every interval and subsequently renal
sections were obtained for the evaluation of tubular and glomerular regeneration
and proliferation. Cell transplantation resulted in the reduction of tubular and
glomerular atrophy after 2 weeks. The transplanted cells were observed in the
reconstructed region of the kidneys as evidenced by the presence of fluorescently
labeled cells both in tubules and glomeruli. We also observed significant
decrease in interstitial fibrosis in the fourth week and there were higher amount
of Ki-67-positive cells in GIII. Notably, the right renal tissue integrity was
significantly improved in this group and revealed normal cortical function on day
60. Transplanting RPCs showed the potential for partial augmentation of kidney
structure and function in pyelonephritis. Cellular repair was seen predominantly
in the proximal tubule, the major site of injury in pyelonephritis. Our findings
may pave the way toward the future regeneration of renal scarring of
pyelonephritis in children.