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3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human
Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis
#MMPMID27022400
Zhang X
; Hu MG
; Pan K
; Li CH
; Liu R
Stem Cells Int
2016[]; 2016
(?): 4626073
PMID27022400
show ga
Three-dimensional (3D) cell culture has been reported to increase the therapeutic
potentials of mesenchymal stem cells (MSCs). However, the action mechanisms of 3D
MSCs vary greatly and are far from being thoroughly investigated. In this study,
we aimed to investigate the therapeutic effects of 3D spheroids of human
adipose-derived MSCs for hepatic fibrosis. Our results showed that 3D culture
enhanced the expression of antifibrotic factors by MSCs, including insulin growth
factor 1 (IGF-1), interleukin-6 (IL-6), and hepatocyte growth factor (HGF). In
vitro studies indicated conditioned medium of 3D cultured MSCs protected
hepatocytes from cell injury and apoptosis more effectively compared with 2D
cultured cells. More importantly, when transplanted into model mice with hepatic
fibrosis, 3D spheroids of MSCs were more beneficial in ameliorating hepatic
fibrosis and improving liver function than 2D cultured cells. Therefore, the 3D
culture strategy improved the therapeutic effects of MSCs and might be promising
for treatment of hepatic fibrosis.
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