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10.1534/genetics.115.182162

http://scihub22266oqcxt.onion/10.1534/genetics.115.182162
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C4788126!4788126!26953268
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suck abstract from ncbi


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pmid26953268      Genetics 2016 ; 202 (3): 885-901
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  • CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering #MMPMID26953268
  • Dickinson DJ; Goldstein B
  • Genetics 2016[Mar]; 202 (3): 885-901 PMID26953268show ga
  • The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)?Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.
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