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10.1038/mt.2016.1 http://scihub22266oqcxt.onion/10.1038/mt.2016.1 C4786925!4786925!26750397     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Ther 2016 ; 24 (3): 475-87 Nephropedia Template TP {{tp|p=26750397|t=2016. Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing |pdf=|usr=}}{{26750397}} gab.com Text Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing JO: Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=26750397 #FOAvip The rapid advancement in targeted genome editing using engineered nucleases such as ZFNs, TALENs, and CRISPR/Cas9 systems has resulted in a suite of powerful methods that allows researchers to target any genomic locus of interest. A complementary set of design tools has been developed to aid researchers with nuclease design, target site selection, and experimental validation. Here, we review the various tools available for target selection in designing engineered nucleases, and for quantifying nuclease activity and specificity, including web-based search tools and experimental methods. We also elucidate challenges in target selection, especially in predicting off-target effects, and discuss future directions in precision genome editing and its applications. Twit Text FOAVip Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing ????Mol Ther http://www.kidney.de/pget.php?&tw=1&pmid=26750397 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26750397 #FOAvip English Wikipedia <ref>{{Cite pmid|26750397}}</ref> Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing #MMPMID26750397 Lee CM; Cradick TJ; Fine EJ; Bao G Mol Ther 2016[Mar]; 24 (3): 475-87 PMID26750397show ga The rapid advancement in targeted genome editing using engineered nucleases such as ZFNs, TALENs, and CRISPR/Cas9 systems has resulted in a suite of powerful methods that allows researchers to target any genomic locus of interest. A complementary set of design tools has been developed to aid researchers with nuclease design, target site selection, and experimental validation. Here, we review the various tools available for target selection in designing engineered nucleases, and for quantifying nuclease activity and specificity, including web-based search tools and experimental methods. We also elucidate challenges in target selection, especially in predicting off-target effects, and discuss future directions in precision genome editing and its applications. äNuclease Target Site Selection for Maximizing On-target Activity and M(epmc).pdf DeepDyve Pubget Overpricing