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10.1038/ncomms10934 http://scihub22266oqcxt.onion/10.1038/ncomms10934 C4786874!4786874!26956543     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=26956543|t=2016. Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system |pdf=|usr=}}{{26956543}} gab.com Text Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26956543 #FOAvip Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes. By delivering Cas9 and guide-RNA (gRNA) with retro- or lenti-virus to IgM+ mouse B cells and hybridomas, we induce class-switch recombination (CSR) of the IgH chain to the desired subclass. Similarly, we induce CSR in all human B cell lines tested with high efficiency to targeted IgH subclass. Finally, we engineer mouse hybridomas to secrete Fab? fragments instead of the whole Ig. Our results indicate that Ig genes in mouse and human cells can be edited to obtain any desired IgH switching helpful to study the biology of normal and lymphoma B cells. We also propose applications that could transform the technology of antibody production. Twit Text FOAVip Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=26956543 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26956543 #FOAvip English Wikipedia <ref>{{Cite pmid|26956543}}</ref> Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system #MMPMID26956543 Cheong TC; Compagno M; Chiarle R Nat Commun 2016[]; 7 (ä): ä PMID26956543show ga Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes. By delivering Cas9 and guide-RNA (gRNA) with retro- or lenti-virus to IgM+ mouse B cells and hybridomas, we induce class-switch recombination (CSR) of the IgH chain to the desired subclass. Similarly, we induce CSR in all human B cell lines tested with high efficiency to targeted IgH subclass. Finally, we engineer mouse hybridomas to secrete Fab? fragments instead of the whole Ig. Our results indicate that Ig genes in mouse and human cells can be edited to obtain any desired IgH switching helpful to study the biology of normal and lymphoma B cells. We also propose applications that could transform the technology of antibody production. äEditing of mouse and human immunoglobulin genes by CRISPR-Cas9 system (epmc).pdf DeepDyve Pubget Overpricing