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10.1016/j.jviromet.2014.09.020 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2014.09.020 C4786303!4786303!25286177     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2015 ; 211 (ä): 64-9 Nephropedia Template TP {{tp|p=25286177|t=2015. Sequencing Human Rhinoviruses: Direct sequencing versus plasmid cloning |pdf=|usr=}}{{25286177}} gab.com Text Sequencing Human Rhinoviruses: Direct sequencing versus plasmid cloning JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=25286177 #FOAvip Human rhinoviruses (RV) are associated with the majority of viral respiratory illnesses in infants, children and adults. Over the last several years, researchers have begun to sequence the many different species and strains of RV in order to determine if certain species were associated with increased disease severity. There are a variety of techniques employed to prepare samples for sequencing. One method utilizes plasmid-cloning, which is expensive and takes several hours to complete. Recently, some investigators have instead used direct sequencing to sequence RV strains, allowing for omission of the time- and labor-intensive cloning step. This study formally compares and contrasts the sequencing results obtained from plasmid-cloning and direct Sanger sequencing of a 500 base pair PCR product covering the VP4/VP2 region of RV. A slightly longer sequence (by 65 base pairs on average) was obtained when specimens were plasmid cloned, and the sequences were 86% similar. After trimming the extra base pairs from the cloned sequences, the sequences were 99.7% identical. Overall success of directly sequencing samples was similar to that of cloning, 5% on average failed for each technique. Therefore, in many instances, directly sequencing samples may be considered in lieu of the more expensive and time-consuming plasmid-cloning technique. Twit Text FOAVip Sequencing Human Rhinoviruses: Direct sequencing versus plasmid cloning ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=25286177 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25286177 #FOAvip English Wikipedia <ref>{{Cite pmid|25286177}}</ref> Sequencing Human Rhinoviruses: Direct sequencing versus plasmid cloning #MMPMID25286177 Linder JE; Plachco TE; Libster R; Miller EK J Virol Methods 2015[Jan]; 211 (ä): 64-9 PMID25286177show ga Human rhinoviruses (RV) are associated with the majority of viral respiratory illnesses in infants, children and adults. Over the last several years, researchers have begun to sequence the many different species and strains of RV in order to determine if certain species were associated with increased disease severity. There are a variety of techniques employed to prepare samples for sequencing. One method utilizes plasmid-cloning, which is expensive and takes several hours to complete. Recently, some investigators have instead used direct sequencing to sequence RV strains, allowing for omission of the time- and labor-intensive cloning step. This study formally compares and contrasts the sequencing results obtained from plasmid-cloning and direct Sanger sequencing of a 500 base pair PCR product covering the VP4/VP2 region of RV. A slightly longer sequence (by 65 base pairs on average) was obtained when specimens were plasmid cloned, and the sequences were 86% similar. After trimming the extra base pairs from the cloned sequences, the sequences were 99.7% identical. Overall success of directly sequencing samples was similar to that of cloning, 5% on average failed for each technique. Therefore, in many instances, directly sequencing samples may be considered in lieu of the more expensive and time-consuming plasmid-cloning technique. äSequencing Human Rhinoviruses: Direct sequencing versus plasmid clonin(epmc).pdf DeepDyve Pubget Overpricing