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10.1038/nbt.3326 http://scihub22266oqcxt.onion/10.1038/nbt.3326 C4785103!4785103!26280411     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2015 ; 33 (9): 952-61 Nephropedia Template TP {{tp|p=26280411|t=2015. Massively parallel high-order combinatorial genetics in human cells |pdf=|usr=}}{{26280411}} gab.com Text Massively parallel high-order combinatorial genetics in human cells JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=26280411 #FOAvip The systematic functional analysis of combinatorial genetics has been limited by the throughput that can be achieved and the order of complexity that can be studied. To enable massively parallel characterization of genetic combinations in human cells, we developed a technology for rapid, scalable assembly of high-order barcoded combinatorial genetic libraries that can be quantified with high-throughput sequencing. We applied this technology, combinatorial genetics en masse (CombiGEM), to create high-coverage libraries of 1,521 two-wise and 51,770 three-wise barcoded combinations of 39 human microRNA (miRNA) precursors. We identified miRNA combinations that synergistically sensitize drug-resistant cancer cells to chemotherapy and/or inhibit cancer cell proliferation, providing insights into complex miRNA networks. More broadly, our method will enable high-throughput profiling of multifactorial genetic combinations that regulate phenotypes of relevance to biomedicine, biotechnology and basic science. Twit Text FOAVip Massively parallel high-order combinatorial genetics in human cells ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=26280411 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26280411 #FOAvip English Wikipedia <ref>{{Cite pmid|26280411}}</ref> Massively parallel high-order combinatorial genetics in human cells #MMPMID26280411 Wong ASL; Choi GCG; Cheng AA; Purcell O; Lu TK Nat Biotechnol 2015[Sep]; 33 (9): 952-61 PMID26280411show ga The systematic functional analysis of combinatorial genetics has been limited by the throughput that can be achieved and the order of complexity that can be studied. To enable massively parallel characterization of genetic combinations in human cells, we developed a technology for rapid, scalable assembly of high-order barcoded combinatorial genetic libraries that can be quantified with high-throughput sequencing. We applied this technology, combinatorial genetics en masse (CombiGEM), to create high-coverage libraries of 1,521 two-wise and 51,770 three-wise barcoded combinations of 39 human microRNA (miRNA) precursors. We identified miRNA combinations that synergistically sensitize drug-resistant cancer cells to chemotherapy and/or inhibit cancer cell proliferation, providing insights into complex miRNA networks. More broadly, our method will enable high-throughput profiling of multifactorial genetic combinations that regulate phenotypes of relevance to biomedicine, biotechnology and basic science. äMassively parallel high-order combinatorial genetics in human cells (epmc).pdf DeepDyve Pubget Overpricing