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10.1111/jth.13239 http://scihub22266oqcxt.onion/10.1111/jth.13239 C4785059!4785059!26712312     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Thromb+Haemost 2016 ; 14 (3): 551-8 Nephropedia Template TP {{tp|p=26712312|t=2016. Neutrophil elastase-deficient mice form neutrophil extracellular traps in an experimental model of deep vein thrombosis |pdf=|usr=}}{{26712312}} gab.com Text Neutrophil elastase-deficient mice form neutrophil extracellular traps in an experimental model of deep vein thrombosis JO: J Thromb Haemost http://www.kidney.de/pget.php?&tw=1&pmid=26712312 #FOAvip Background: Neutrophil serine proteases have been implicated in coagulation and neutrophil extracellular trap (NET) formation. In human neutrophils, neutrophil elastase (NE) translocates to the nucleus during NETosis and cleaves histones, thus aiding in chromatin decondensation. NE?/? mice were shown not to release NETs in response to microbes. However, mouse studies evaluating the role of NE in NET formation in sterile inflammation and thrombosis are lacking. Objective: We wished to establish if neutrophils from NE?/? mice have a defect in NETosis, similar to peptidylarginine deiminase 4 (PAD4?/?) mice, and how this might impact venous thrombosis, a model where NETs are produced and are crucial to thrombus development. Methods: We performed in vitro NET assays using neutrophils from wild-type (WT), NE?/?, SerpinB1 (SB1?/?), and NE?/?SB1?/? mice. We compared WT and NE?/? animals in the inferior vena cava stenosis model of deep vein thrombosis (DVT). Results: NE-deficiency resulted in a small reduction in ionomycin-induced NET formation in vitro without affecting histone citrullination. However, NET production in response to PMA or PAF was normal in neutrophils from two independent NE-deficient mouse lines, or in NE?/?SB1?/? as compared to SB1?/? neutrophils. NE-deficiency or inhibition did not prevent NETosis in vivo and DVT outcome. Conclusions: NE is not required for NET formation in mice. NE?/? mice, which form pathological venous thrombi containing NETs, do not phenocopy PAD4?/? mice in in vitro NETosis assays or experimental venous thrombosis. Our study suggests that NET-targeted therapies need to be highly effective to have an impact on DVT. Twit Text FOAVip Neutrophil elastase-deficient mice form neutrophil extracellular traps in an experimental model of deep vein thrombosis ????J Thromb Haemost http://www.kidney.de/pget.php?&tw=1&pmid=26712312 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26712312 #FOAvip English Wikipedia <ref>{{Cite pmid|26712312}}</ref> Neutrophil elastase-deficient mice form neutrophil extracellular traps in an experimental model of deep vein thrombosis #MMPMID26712312 Martinod K; Witsch T; Farley K; Gallant M; Remold-O?Donnell E; Wagner DD J Thromb Haemost 2016[Mar]; 14 (3): 551-8 PMID26712312show ga Background: Neutrophil serine proteases have been implicated in coagulation and neutrophil extracellular trap (NET) formation. In human neutrophils, neutrophil elastase (NE) translocates to the nucleus during NETosis and cleaves histones, thus aiding in chromatin decondensation. NE?/? mice were shown not to release NETs in response to microbes. However, mouse studies evaluating the role of NE in NET formation in sterile inflammation and thrombosis are lacking. Objective: We wished to establish if neutrophils from NE?/? mice have a defect in NETosis, similar to peptidylarginine deiminase 4 (PAD4?/?) mice, and how this might impact venous thrombosis, a model where NETs are produced and are crucial to thrombus development. Methods: We performed in vitro NET assays using neutrophils from wild-type (WT), NE?/?, SerpinB1 (SB1?/?), and NE?/?SB1?/? mice. We compared WT and NE?/? animals in the inferior vena cava stenosis model of deep vein thrombosis (DVT). Results: NE-deficiency resulted in a small reduction in ionomycin-induced NET formation in vitro without affecting histone citrullination. However, NET production in response to PMA or PAF was normal in neutrophils from two independent NE-deficient mouse lines, or in NE?/?SB1?/? as compared to SB1?/? neutrophils. NE-deficiency or inhibition did not prevent NETosis in vivo and DVT outcome. Conclusions: NE is not required for NET formation in mice. NE?/? mice, which form pathological venous thrombi containing NETs, do not phenocopy PAD4?/? mice in in vitro NETosis assays or experimental venous thrombosis. Our study suggests that NET-targeted therapies need to be highly effective to have an impact on DVT. äNeutrophil elastase-deficient mice form neutrophil extracellular traps(epmc).pdf DeepDyve Pubget Overpricing